中国生物防治学报2025，Vol.41Issue(3)：542-553,12.DOI:10.16409/j.cnki.2095-039x.2025.02.015
生防菌Pc01-6对丹参根腐病的生防效果及基因组学分析
Biocontrol Effects of Antagonistic Isolate Pc01-6 on Salvia miltiorrhiza Root Rot and Its Genomic Analysis
摘要
Abstract
In this study,an effective antagonistic isolate,Pc01-6,was isolated and screened from the rhizosphere soil of Salvia miltiorrhiza using the plate confrontation method to explore superior bioncontrol resources against S.miltiorrhiza root rot.The isolate exhibited an inhibition rate of 63.67%to 68.40%against the root rot pathogen,and demonstrated broad-spectrum antagonistic activity against 10 other plant pathogenic fungi.Based on morphological,physiological and biochemical characteristics,as well as 16S rDNA and gyrB sequence analysis,the isolate Pc01-6 was identified as Pseudomonas chlororaphis subsp.Aurantiaca.Pot experiments revealed that the isolate provided a control efficacy of 53.30%to 65.22%against S.miltiorrhiza root rot,while significantly promoting plant growth,with increases of 35.10%and 72.34%in fresh stem and leaf weight and root weights,respectively.Field trials further confirmed its efficiency,showing a control rate of 50.91%against root rot.Whole-genome sequencing analysis indicated that the genome of Pc01-6 is 6,835,850 bp in size,with a GC content of 62.87%,and encodes 6,159 genes.Function gene prediction revealed the presence of gene clusters associated with the biosynthesis of secondary metabolites with antibacterial activity,such as cyclolipopeptides and pyrrolnitrin.In summary,isolate Pc01-6 demonstrates significant biocontrol effects against S.miltiorrhiza root rot and holds promise for practical applications.关键词
丹参根腐病/拮抗菌株/绿针假单胞菌桔黄亚种/全基因组测序/次生代谢产物生物合成基因簇Key words
Salvia miltiorrhiza root rot/antagonistic isolate/Pseudomonas chlororaphis subsp.Aurantiaca/whole genome sequencing/secondary metabolite biosynthesis gene cluster分类
农业科技引用本文复制引用
王飞,宋露洋,杨健,万修福,李绍建,李雪梦,杨瑾,高素霞,郭治辰,秦艳红,文艺,鲁书豪,赵莹..生防菌Pc01-6对丹参根腐病的生防效果及基因组学分析[J].中国生物防治学报,2025,41(3):542-553,12.基金项目
国家中药材产业技术体系(CARS-21) （CARS-21）
河南省中药材产业技术体系(HARS-22-11-Z1) （HARS-22-11-Z1）
河南省农科院新兴学科(2024XK06) （2024XK06）
河南省农业科学院自主创新项目(2025ZC51) （2025ZC51）
河南省科技攻关项目(252102111102) （252102111102）
