中国生物防治学报2025，Vol.41Issue(3)：554-560,7.DOI:10.16409/j.cnki.2095-039x.2025.02.024
黄芪根腐病生防放线菌的筛选、鉴定及其应用
Screening,Identification,and Application Effects of Actinomycetes on Astragalus membranaceus Root Rot
摘要
Abstract
To provided biocontrol germplasm resources for root rot disease in traditional Chinese medicine,screening,identification,and application evaluation of Streptomyces,an actinomycete with significant antibacterial effects against various Fusarium species,were carried out.The strain LQS-2 with significant antibacterial effect against Astragalus membranaceus root rot disease was screened from 120 strains of actinomycetes,and against the pathogenic bacterium F.moniliforme,F.solani N18-1-2,F.verticillium 173,and F.oxysporum 295,the antibacterial rates each for 62.50%,49.38%,52.75%,and 67.50%.16S rDNA sequence analysis and alignment showed that strain LQS-2 had the highest homology with the gene sequence of Streptomyces cellulosae,and combined with morphology,strain LQS-2 was determined to be S.cellulosae.The results of pot experiment showed that the greenhouse prevention effect of soaking the fermentation supernatant of S.cellulosiae LQS-2 combined with root irrigation on Astragalus membranaceus root rot disease was 69.50%.The field prevention effect of continuous root irrigation three times on Astragalus membranaceus root rot disease reached 76.02%,which was 2.55%and 18.76%higher than the control effect of microbial agents.After soaking in strain LQS-2,it showed a significant growth promoting effect on Astragalus membranaceus seedlings under greenhouse conditions.The root length,biomass,and underground dry weight increased by 37.58%,20.00%and 33.33%,which compared to microbial agents.From this,it could be seen that S.cellulosiae LQS-2 has the potential to be developed as a biocontrol agent for the prevention and treatment of Astragalus membranaceus root rot disease.关键词
黄芪根腐病/纤维素链霉菌/致病镰孢菌/防治效果Key words
Astragalus membranaceus root rot/Streptomyces cellulosae/pathogenic Fusarium/prevention and control effect分类
农业科技引用本文复制引用
杨波,李云翔,徐瑾瑜,沙月霞..黄芪根腐病生防放线菌的筛选、鉴定及其应用[J].中国生物防治学报,2025,41(3):554-560,7.基金项目
宁夏重点研发计划(2022BBF02031) （2022BBF02031）
宁夏农林科学院科技攻关项目(NKYG-24-20) （NKYG-24-20）
