中国生物防治学报2025，Vol.41Issue(3)：561-570,10.DOI:10.16409/j.cnki.2095-039x.2025.02.031
当归枯萎病拮抗菌的鉴定及促生防病效果
Identification of Antagonistic Bacteria against Angelica Wilt and Their Effect on Promoting Growth and Disease Controlling
摘要
Abstract
To clarify the classification status of antagonistic bacteria DST27 and HQT43 against Angelica wilt,evaluate their biocontrol effects,and provide theoretical basis for their development and utilization.The strain was identified using morphological characterization,physiological and biochemical traits,and 16S rDNA sequencing analysis.The potential of biocontrol bacteria to promote the growth of Angelica sinensis,prevent the illness known as Angelica wilt,and influence the kinds and numbers of soil microorganisms in the plant's rhizosphere were all investigated through pot experiments.In the early stage,strains DST27 and HQT43 with phosphorus solubilization,nitrogen fixation,and antibacterial functions were isolated from the rhizosphere soil of Codonopsis pilosula and Astragalus,preliminary identification of strain DST27 as Bacillus pumilus and HQT43 as Streptomyces albidoflavus.Significant differences(P＜0.05)were observed in the promotion effects of DST27 and its mixed inoculant(DST27:HQT43=1:1)on stem diameter,fresh weight,dry weight of aboveground part,fresh weight,and dry weight of underground portion.The findings demonstrated that the biocontrol agents'ability to prevent Angelica wilt outweighed their ability to treat it.The prevention effects of DST27,HQT43,and DST27+HQT43(1:1)on Angelica wilt were 71.88%,43.75%,and 73.53%,respectively,in the preventative group.The prevention and treatment effects in the treatment group were 65.62%,34.38%,and 43.75%,in that order.Rhizosphere bacteria and actinomycetes dramatically increased following treatment with biocontrol bacteria,whereas the number of fungus significantly decreased.Actinomycetes and fungi in rhizosphere soil were most significantly affected by the mixed application of DST27+HQT43 at a 1:1 ratio.A good development and application prospect is presented by the combined application of DST27 and HQT43 in a 1:1 ratio,which may clearly alter the population of soil microorganisms and encourage crop growth in addition to having a good biocontrol effect.关键词
当归枯萎病/短小芽胞杆菌DST27/微白黄链霉菌HQT43/菌种鉴定/生物防治Key words
Angelica wilt disease/Bacillus pumilus DST27/Streptomyces albidoflavus HQT43/strain identification/biocontrol分类
农业科技引用本文复制引用
李铮,王允瑶,郭增祥,栾涵钰,付瑶,王伟,晋玲,王艳..当归枯萎病拮抗菌的鉴定及促生防病效果[J].中国生物防治学报,2025,41(3):561-570,10.基金项目
甘肃省自然科学基金(25JRRA933) （25JRRA933）
甘肃省科技计划(23ZDFA013-1) （23ZDFA013-1）
西北中藏药省部共建协同创新中心开放基金(Xbzzy-2022-02) （Xbzzy-2022-02）
国家现代农业产业技术体系资助(CARS-21) （CARS-21）
甘肃省2023年度优秀研究生"创新之星"项目(2023CXZX-763) （2023CXZX-763）
