中国生物防治学报2025，Vol.41Issue(3)：585-592,8.DOI:10.16409/j.cnki.2095-039x.2025.02.038
解淀粉芽胞杆菌ZF522的分离鉴定及对生姜根腐病防治效果
Isolation and Identification of Bacillus amyloliquefaciens ZF522 and Its Control Effect on Ginger Root Rot
摘要
Abstract
Ginger root rot caused by Pythium aphanidermatum seriously endangers ginger production and brings serious economic losses to ginger production.At present,the stem base diseases of ginger are difficult to control,and the use of chemical agents is easy to remain.The biological control of rhizome diseases is also lack of effective prevention and control products.In this experiment,Bacillus was isolated from the rhizosphere soil of ginger,and a strain with good control effect was screened by in vitro screening of ginger root rot.ZF522 was identified as Bacillus amyloliquefaciens according to its morphological characteristics,physiological and biochemical characteristics and phylogenetic tree analysis.Under the condition of pot experiment,the control effect of biocontrol strain ZF522 on ginger root rot reached 50.84%.Agarose gel electrophoresis showed that strain ZF522 contained srfA,fenA,ituA,ituC,bymA,bymB and other antimicrobial lipopeptide synthesis genes.These genes are closely related to the reported antimicrobial lipopeptides.In summary,strain ZF522 has good biocontrol potential against ginger root rot,and is a biocontrol strain with development and utilization value.关键词
解淀粉芽胞杆菌/生姜根腐病/拮抗菌/菌株鉴定/防治效果Key words
Bacillus amyloliquefaciens/ginger rhizome rot/antagonistic bacterium/strain identification/control effect分类
农业科技引用本文复制引用
李森,李磊,刘世程,段广荣,陈朱侃,石延霞,柴阿丽,李宝聚,谢学文..解淀粉芽胞杆菌ZF522的分离鉴定及对生姜根腐病防治效果[J].中国生物防治学报,2025,41(3):585-592,8.基金项目
国家重点研发计划(2024YFD2000010) （2024YFD2000010）
西藏自治区科技计划(XZ202401ZY0049) （XZ202401ZY0049）
中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS) （CAAS-ASTIP-IVFCAAS）
农业农村部园艺作物生物学与种质创制重点实验室(IVF2023) （IVF2023）
