中国生物防治学报2025，Vol.41Issue(3)：593-604,12.DOI:10.16409/j.cnki.2095-039x.2025.02.033
烟草靶斑病拮抗菌株MLY-3的筛选及发酵条件优化
Screening of Antagonistic Strain MLY-3 against Tobacco Target Spot and Its Optimization of Fermentation Conditions
摘要
Abstract
Tobacco target spot is an important leaf disease that endangers tobacco production,and it is important to screen biocontrol strains against R.solani and optimize the fermentation conditions of the strains.To control tobacco target spot,biocontrol strains were isolated from the rhizosphere soil of healthy tobacco by dilution coating plate method and plate confrontation test.Strain MLY-3 with 80.92%inhibition rate against R.solani was screened out.Microscopic examination revealed that strain MLY-3 induced distortion and deformity of the mycelium during confrontation culture.Strain MLY-3 was identified as P.aeruginosa by morphological observation,physiological and biochemical detection and molecular analysis.Single factor test and orthogonal test results showed that the optimal fermentation medium components of strain MLY-3 were as follows:mannitol 10 g/L,beef extract 5 g/L and MgCl2 7.5 g/L.The optimal fermentation conditions were as follows:initial pH 5.5,inoculum 3%,temperature 30℃and time 36 h.The inhibition rate of its aseptic fermentation broth against R.solani was increased by 53.8%under optimal fermentation conditions.The field control effect of strain MLY-3 fermentation broth against tobacco target spot was 70.03%,indicating great potential for biocontrol application.This study enriched the biocontrol resource of tobacco target spot and laid the foundation for its application.关键词
烟草靶斑病/铜绿假单胞菌/发酵条件优化/防治效果Key words
tobacco target spot/Pseudomonas aeruginosa/optimization of fermentation conditions/control effect分类
农业科技引用本文复制引用
向丹东,彭静,刘天波,唐前君,肖钦之,王灿,邓征宇,刘峰,肖志鹏,熊有明,王冰,梅斯钇..烟草靶斑病拮抗菌株MLY-3的筛选及发酵条件优化[J].中国生物防治学报,2025,41(3):593-604,12.基金项目
湖南省重点领域研发计划(2023NK2019) （2023NK2019）
湖南省烟草公司永州市公司科技项目(YZ2023KJ02) （YZ2023KJ02）
中国烟草总公司项目(110202101050(LS-10),110202201019(LS-03),110202201027(LS-11)) （110202101050(LS-10）
湖南省烟草公司衡阳市公司项目(HYYC2023KJ32) （HYYC2023KJ32）
湖南省农业科技创新资金项目(2023CX40) （2023CX40）
长沙市自然科学基金(kq2208072) （kq2208072）
