中国生物防治学报2025，Vol.41Issue(3)：605-616,12.DOI:10.16409/j.cnki.2095-039x.2025.02.032
13种植物提取物对3种小麦病原菌的抑菌活性筛选及其成分分析
Screening of Antifungal Activity of Extracts from 13 species of Plant against Three Wheat Pathogens and Analysis of Their Constituents
摘要
Abstract
Screening and evaluating the antibacterial activity of Xinjiang's plant resources provides a foundation for the development of botanical pesticides.In this study,the antifungal activities of 13 plant extracts from Xinjiang were screened and measured through the mycelial growth rate method,targeting three wheat pathogens:Bipolaris sorokiniana,Fusarium graminearum,and Microdochium nivale.Additionally,the main components of Rumex crispus were analyzed using ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The organic solvent extracts from 13 plant species showed different inhibitory activities against three wheat pathogenic fungi,and the antibacterial efficacy increased with increasing concentration.Among them,the methanol extract of Rumex crispus exhibited the strongest inhibitory activity against B.sorokiniana,with an EC50 value of 6.380 mg/mL;the methanol extract of Salsola collina demonstrated the strongest inhibitory activity against F.graminearum,with an EC50 value of 26.362 mg/mL;and the ethanol extract of R.crispus showed the strongest inhibitory activity against Microdochium nivale,with an EC50 value of 8.203 mg/mL.A total of 42 chemical components were identified in the R.crispus extract,primarily flavonoids,fatty acyls,carboxylic acids,and their derivatives,accounting for 66.67%of the total.It can be seen from this that the methanol extract of R.crispus has good antibacterial activity and is worth further study.关键词
植物提取物/小麦病原菌/抑菌活性/超高效液相色谱串联质谱(UPLC-MS/MS)Key words
plant extracts/wheat pathogenic bacteria/antibacterial activity/ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)分类
医药卫生引用本文复制引用
颜彬,梁晶,龚静云,周小云,胡林峰,张军高,雷斌,李克梅..13种植物提取物对3种小麦病原菌的抑菌活性筛选及其成分分析[J].中国生物防治学报,2025,41(3):605-616,12.基金项目
新疆维吾尔自治区中央引导地方科技发展专项资金项目(ZYYD2024JD18) （ZYYD2024JD18）
中央引导地方项目"'一带一路'国际绿色农药研制及应用技术创新基地建设"(ZYYD2023B06) （ZYYD2023B06）
自治区科技特派员农村科技创业行动项目"春小麦高产创建关键技术集成与示范(2022BKZ022)" （2022BKZ022）
