中国输血杂志2025，Vol.38Issue(7)：867-872,6.DOI:10.13303/j.cjbt.issn.1004-549x.2025.07.001
纳米血红蛋白氧载体对肺癌细胞系放疗敏感性的影响
Effect of nano hemoglobin-based oxygen carrier on radiosensitivity of lung cancer cell line
刘长民 1李勇 1李凤娟 2周文涛 3黄炳镠 3刘嘉馨 3杨成民1
作者信息
- 1. 中国医学科学院输血研究所,四川 成都 610052||天津协和生物科技有限公司,天津 300457
- 2. 天津工业大学,天津 300387
- 3. 中国医学科学院输血研究所,四川 成都 610052
- 折叠
摘要
Abstract
Objective To study the effect of nano hemoglobin-based oxygen carrier(nano-HBOC)on radiosensitivity of lung cancer H385 cells.Methods Using 95%N2 and 5%CO2,a lung cancer cell line was constructed in a hypoxic envi-ronment,and H385 cells were treated with different concentrations of nano-HBOC and irradiated(4Gy)by an irradiator,and the IC50 concentration was calculated.The cells were detected by flow cytometry(reactive oxygen species,ROS)ROS test.Using GEO database,KEGG pathway enrichment analysis was carried out to predict possible pathways.The levels of lipid peroxidation and Fe2+were observed by fluorescence microscope,and the proteins related to iron death pathway were detected by Western-blot.Results Compared with the control cells,the activity and density of the cells were significantly decreased by nano-HBOC combined with radiotherapy,with a notable proportion of cells exhibiting deteriorated status.There is a positive correlation between ROS level and nano-HBOC concentration,especially after radiotherapy.Radiotherapy combined with nano-HBOC significantly increased the levels of lipid peroxidation and Fe2+in H385 cells,while decreasing the levels of iron death pathway proteins slc7a11 and GPX4,and increasing the level of ACSL4.Conclusion Nano-HBOC enhances the radiosensitivity of lung cancer H385 cells.关键词
纳米血红蛋白氧载体/肺癌/放疗/氧增敏Key words
nano hemoglobin-based oxygen carrier(nano-HBOC)/lung cancer/radiotherapy/oxygen sensitization分类
医药卫生引用本文复制引用
刘长民,李勇,李凤娟,周文涛,黄炳镠,刘嘉馨,杨成民..纳米血红蛋白氧载体对肺癌细胞系放疗敏感性的影响[J].中国输血杂志,2025,38(7):867-872,6.
