中国兽医科学2025，Vol.55Issue(7)：877-883,7.DOI:10.16656/j.issn.1673-4696.2025.0125
猪流行性腹泻病毒变异毒株与S-indel流行毒株S基因嵌合毒株的构建
Construction of a chimeric porcine epidemic diarrhea virus variant strain by replacement of S gene of S-indel prevalent strain
摘要
Abstract
Abstact:In this study,two specifically sgRNAs were designed and utilized to cleave the full-gene BAC recombinant plasmid of AH2012/12.A recombinant BAC plasmid was obtained by transforming the S gene recombinant plasmid of the S-indel strain,and the AH2012/12 gene BAC plasmid into NEB 10-beta E.coli.Subsequently,transfection of the recombinant BAC plasmid into Vero cells resulted in the rescue of a chimeric AH2012/12 recombinant virus strain,wherein the S gene was replaced with that of the S-indel strain.Viral genome sequencing analysis,indirect immunofluorescence assays,plaque assays,and growth curve measurements collectively confirmed the successful rescue of this chimeric virus,designated as rAH2012/12-S.It lays an important foundation for the development of novel PEDV vaccines,and enables a more rapid response to the prevention and control of emerging PEDV variants in the future.关键词
猪流行性腹泻病毒/S基因/反向遗传Key words
porcine epidemic diarrhea virus/S gene/reverse genetics分类
农业科技引用本文复制引用
尹丽萍,常长琳,范宝超,丁小燕,白娟,张海涛,李彬,姜平..猪流行性腹泻病毒变异毒株与S-indel流行毒株S基因嵌合毒株的构建[J].中国兽医科学,2025,55(7):877-883,7.基金项目
国家生猪产业技术体系专项(CARS-35) （CARS-35）
江苏省自主创新研发基金项目(CX(22)1011) （CX(22）
