中国兽医科学2025，Vol.55Issue(7)：884-890,7.DOI:10.16656/j.issn.1673-4696.2025.0116
可表达禽腺病毒Fiber2基因的重组马立克病病毒疫苗株的构建
Construction of a recombinant Marek's disease virus vaccine strain expressing the Fiber2 gene of fowl adenovirus
摘要
Abstract
To develop a recombinant Marek's disease virus(MDV)live vector vaccine for avian hepati-tis-hydropericardium syndrome(HHS),the Fiber2 gene was amplified from a virulent serotype 4 fowl adeno-virus(FAdV-4)strain using PCR and cloned into the pCDNA3.1 vector to construct the recombinant eukary-otic expression plasmid pSV40-Fiber2.Subsequently,the expression fosmid pMS-Fiber2 was constructed through PCR and homologous recombination.This fosmid,along with four other overlapping plasmids covering the entire MDV genome,was co-transfected into chicken embryo fibroblast(CEF)cells to rescue the recombinant virus rMDV-Fiber2.Following serial passages in CEF,rMDV-Fiber2 was identified using PCR,indirect immunofluorescence assay and Western-blot,and its in vitro replication characteristics were analyzed by growth curve plotting.The results indicated that rMDV-Fiber2 stably expressed the Fiber2 protein and exhibited same replication capacity in CEF comparable to the parental virus rMSΔmeq.Challenge experiments demonstrated that chickens immunized with rMDV-Fiber2 achieved an 80％survival rate when challenged with the virulent FAdV-4 strain.In conclusion,the replication ability of the successful constructed rMDV-Fiber2 was similar to that of the parental strain,which provided a foun-dation for developing a recombinant MDV live vector vaccine for HHS.关键词
血清4型禽腺病毒/重组马立克病病毒活载体疫苗/Fiber2Key words
serotype 4 fowl adenovirus/recombinant Marek's disease virus live vector vaccine/Fiber2分类
农业科技引用本文复制引用
张子博,刘永振,王素艳,崔红玉,段雨路,李留安,高玉龙,陈运通,范文瑞,吴龙波,李雨萌,郭茹,张艳萍,刘长军,祁小乐..可表达禽腺病毒Fiber2基因的重组马立克病病毒疫苗株的构建[J].中国兽医科学,2025,55(7):884-890,7.基金项目
国家自然科学基金项目(U21A20260) （U21A20260）
国家肉鸡产业技术体系项目(CARS-41) （CARS-41）
