中国兽医科学2025，Vol.55Issue(7)：919-924,6.DOI:10.16656/j.issn.1673-4696.2025.0114
口蹄疫病毒146S抗原特异性抗体的制备及生物学特性分析
Preparation of specific antibody against foot-and-mouth disease virus 146S antigen and analysis of its biological characteristics
摘要
Abstract
In order to optimize the preparation process of foot-and-mouth disease(FMD)nanoantibody M170 and M8 proteins,recombinant expression plasmids were constructed to express the two recombinant proteins by E.coli or HEK293 cells,respectively.The purity,yield and affinity of M170 and M8 were com-pared and analyzed.The results showed that the yield of M170 and M8 prepared by prokaryotic expression system was 2.5～3 times than that of eukaryotic expression system,and the production cycle of prokaryotic expression system could be shortened to 2 days,which was suitable for large-scale production,but the preparation of M170 and M8 by eukaryotic expression systems need at least 7 days and yields were low.the results of protein activity test showed that there was no significant difference in conformation between M170 and M8 prepared by the two expression systems,and both could specifically recognize foot-and-mouth disease virus 146S antigen or 12S antigen.This study provided a reference for the production process opti-mization of 0-type FMD antigen quantitative ELISA kit.关键词
口蹄疫病毒/146S抗原/纳米抗体Key words
foot-and-mouth disease virus/146S anti gen/nano-antibody分类
农业科技引用本文复制引用
刘仲元,南栩,谭书桢,秦峰,董虎,张韵,郭慧琛,孙世琪,徐业芬..口蹄疫病毒146S抗原特异性抗体的制备及生物学特性分析[J].中国兽医科学,2025,55(7):919-924,6.基金项目
甘肃省自然科学基金项目(24JRRA012) （24JRRA012）
国家自然科学基金青年基金项目(32301127) （32301127）
2023兰州市人才创业创新项目(2023-RC-3) （2023-RC-3）
