中国兽医杂志2025，Vol.61Issue(7)：63-68,6.DOI:10.20157/j.cnki.zgsyzz.2025.07.007
水禽细小病毒LAMP及其可视化通用检测方法的建立
Establishment of LAMP and Visual General Detection Method for Waterfowl Parvovirus
摘要
Abstract
To develop a universal detection method for goose parvovirus(GPV)and Muscovy duck parvovirus(MDPV),this study designed a set of specific primers targeting the conserved sequence of the NS gene from both GPV and MDPV.A loop-mediated isothermal amplification(LAMP)method and a LAMP-hydroxy naphthol blue(HNB)visual detection method were optimized and established for waterfowl parvovirus detection.The specificity and sensitivity of both methods were evaluated.Additionally,10 clinical waterfowl samples were tested using conventional PCR,LAMP,and LAMP-HNB to assess the practical application of the methods.The results showed that the optimal reaction conditions for the LAMP method were 65℃for 60 minutes.Both methods specifically amplified GPV and MDPV,while no amplification was observed for five other common waterfowl viruses.The minimum detection limit for both methods was 1×10⁻⁵ ng/μL.Moreover,the results of LAMP and LAMP-HNB were 100%consistent with conventional PCR.These findings indicate that the established LAMP and LAMP-HNB visual detection methods exhibit high sensitivity,strong specificity,minimal instrumentation requirements,and easy visualization,making them suitable for rapid on-site detection of waterfowl parvoviruses.关键词
鹅细小病毒(GPV)/番鸭细小病毒(MDPV)/NS基因/环介导等温扩增(LAMP)/可视化Key words
goose parvovirus(GPV)/Muscovy duck parvovirus(MDPV)/NS gene/loop-mediated isothermal amplification(LAMP)/visualization分类
农业科技引用本文复制引用
戴银,赵瑞宏,周学利,侯宏艳,周慧琴,谷思琴,胡晓苗,王洁茹,殷冬冬,尹磊,沈学怀,潘孝成..水禽细小病毒LAMP及其可视化通用检测方法的建立[J].中国兽医杂志,2025,61(7):63-68,6.基金项目
安徽省科技重大专项(202003a06020012) （202003a06020012）
安徽省家禽产业技术体系项目(AHCYJSTX-06) （AHCYJSTX-06）
安徽省农业科学院团队项目(2025YL044) （2025YL044）
