中国现代医学杂志2025，Vol.35Issue(15)：43-51,9.DOI:10.3969/j.issn.1005-8982.2025.15.007
甘露清瘟方通过调控Bcl-2/Bax/Caspase-3信号通路抑制急性肺损伤大鼠肺组织细胞凋亡的机制研究
Ganlu Qingwen formula inhibits apoptosis in lung tissue of acute lung injury rats by regulating the Bcl-2/Bax/Caspase-3 signaling pathway
摘要
Abstract
Objective To investigate the effect of Ganlu Qingwen Formula(GLQWF)on the Bcl-2/Bax/Caspase-3 signaling pathway and its role in inhibiting apoptosis in a rat model of lipopolysaccharide(LPS)-induced acute lung injury(ALI)/acute respiratory distress syndrome(ARDS).Methods Forty-eight male SD rats were randomized into:Blank(saline),Model(LPS),Dexamethasone(Dex,2 mg/kg),and GLQWF Low,Medium,High dose groups(0.6,1.2,2.4 g/kg).Drugs were administered by gavage for 3 days.On day 3,ALI was induced by intratracheal LPS instillation(Blank:saline).Lung histopathology(H&E),serum and lung tissue cytokines(ELISA:TNF-α,IFN-γ,IL-6),and lung tissue apoptosis-related protein(Western blot)and mRNA(qRT-PCR)expression(Bcl-2,Bax,Caspase-3)were analyzed.Results Model group lungs showed extensive consolidation and inflammation.GLQWF(all doses)and Dex significantly reduced serum and lung TNF-α,IFN-γ,IL-6 levels compared to Model(P<0.05).GLQWF(Medium/High doses)and Dex significantly increased Bcl-2 protein/mRNA and decreased Bax/Caspase-3 protein/mRNA expression compared to Model(P<0.05).High-dose GLQWF showed greater effects on protein expression than low-dose.Conclusion Ganlu Qingwen Formula alleviates LPS-induced ALI/ARDS by modulating the Bcl-2/Bax/Caspase-3 pathway,promoting anti-apoptotic Bcl-2 expression,and inhibiting pro-apoptotic Bax and Caspase-3 expression.关键词
急性肺损伤/急性呼吸窘迫综合征/大鼠/甘露清瘟方/细胞凋亡/B淋巴细胞瘤-2/Bcl-2相关X蛋白/半胱氨酸天冬氨酸蛋白酶-3Key words
acute lung injury/acute respiratory distress syndrome/rats/Ganlu Qingwen formula/apoptosis/Bcl-2/Bax/Caspase-3分类
医药卫生引用本文复制引用
李翔鹏,李风森,徐丹,李争,王玲..甘露清瘟方通过调控Bcl-2/Bax/Caspase-3信号通路抑制急性肺损伤大鼠肺组织细胞凋亡的机制研究[J].中国现代医学杂志,2025,35(15):43-51,9.基金项目
"天山英才"医药卫生高层次人才培养计划(No:TSYC202301B048) （No:TSYC202301B048）
新疆维吾尔自治区科学技术厅中医药防治呼吸系统疾病创新团队(No:2023TSYCTD0018) （No:2023TSYCTD0018）
新疆维吾尔自治区重大科技专项项目(No:2022A03006-1). （No:2022A03006-1）
