中国畜牧兽医2025，Vol.52Issue(7)：2965-2980,16.DOI:10.16431/j.cnki.1671-7236.2025.07.001
后备母猪发情期和乏情期下丘脑-垂体-卵巢性腺轴miRNA-mRNA表达谱比较分析
Comparative Analysis of miRNA-mRNA Expression Profile in Hypothalamus-Pituitary-Ovary Gonad Axis During Estrus and Anestrus in Gilts
摘要
Abstract
[Objective]The purpose of this study was to explore the key role of miRNA-mRNA interaction network in the regulation of estrus in gilts for explaining the genetic mechanism of miRNA-mRNA interaction in the estrus activity of gilts.[Method]The hypothalamus,pituitary and ovary of gilts in estrus and anestrus were selected as the study objects.The concentrations of follicle-stimulating hormone(FSH),progesterone(P4),estradiol(E2),and other reproductive hormones in serum were measured.After sRNA-Seq analysis,the differentially expressed miRNA were screened out by bioinformatics software,GO function and KEGG pathway enrichment analysis of differentially expressed miRNA target genes were performed using R language,and 4 differentially expressed miRNAs were verified by Real-time quantitative PCR.[Result]The concentration of reproductive hormone in serum was consistent with the physiological cycle of gilts.A total of 742 known miRNAs and 229 novel miRNAs in gilts were detected during estrus and anestrus,57 differentially expressed miRNAs(24 up-regulated and 33 down-regulated)were found in hypothalamus of gilts during estrus and anestrus,71 differentially expressed miRNAs(44 up-regulated and 27 down-regulated)in pituitary were found,and 140 differentially expressed miRNAs(63 up-regulated and 77 down-regulated)in ovary were found.KEGG pathway enrichment analysis showed that differentially expressed miRNA target genes in hypothalamus of gilts were mainly involved in proteoglycan in cancer,NOD-like receptor signaling pathway,Toll-like receptor signaling pathway,and cytokine receptor interaction.The differentially expressed miRNA target genes in pituitary were mainly involved in glycine,serine and threonine metabolism,GnRH secretion,cell adhesion molecules,metabolic signaling pathway.The differentially expressed miRNA target genes in ovary were mainly involved in chemokine signaling pathway,lysosome,ovarian sterogenesis,cholesterol metabolism,PPAR signaling pathway,and ECM-receptor interaction.In the target gene regulatory network related to reproduction,the key miRNA that might be mediated by hypothalamus-pituitary-ovary gonad axis to regulate the estrus activity of gilts were screened:miR-6240Z,ssc-miR-34a,ssc-miR-143-3P,ssc-miR-127,ssc-miR-21-5P,and ssc-miR-381-3p.Four differentially expressed miRNAs in ovary of gilts were verified by Real-time quantitative PCR,and the expression trends were consistent with the sequencing results.[Conclusion]In this study,miRNA expression profiles of hypothalamus-pituitary-ovary gonad axis of gilts in estrus and anestrus were successfully constructed,and the differentially expressed miRNA was verified.miRNAs that were significantly related to the estrus activity of gilts were screened,which provided theoretical basis for the analysis of the estrus mechanism of gilts.关键词
miRNA-mRNA/后备母猪/发情期/乏情期/下丘脑-垂体-卵巢性腺轴Key words
miRNA-mRNA/gilts/estrus/anestrus/hypothalamus-pituitary-ovary gonad axis分类
农业科技引用本文复制引用
吕玲燕,张冰,蒋家霞,张家庆,孙如玉,林昌华,张胜斌,覃秀珍,柏秀芳,吴永绍,陈钊,刘磊..后备母猪发情期和乏情期下丘脑-垂体-卵巢性腺轴miRNA-mRNA表达谱比较分析[J].中国畜牧兽医,2025,52(7):2965-2980,16.基金项目
国家重点研发项目(2021YFD1301200) （2021YFD1301200）
广西农业职业技术大学校级课题(XKJ2336) （XKJ2336）
广西农业科技自筹经费项目(Z2022115) （Z2022115）
河南省科技攻关项目(242102111030) （242102111030）
广西农业科技自筹经费项目(Z2023101) （Z2023101）
2025年度广西高校中青年教师科研基础能力提升项目(2025KY1388) （2025KY1388）
