中国畜牧兽医2025，Vol.52Issue(7)：3070-3083,14.DOI:10.16431/j.cnki.1671-7236.2025.07.009
1株植物乳杆菌的分离鉴定、生物学特性和安全性研究
Isolation,Identification and Biological Properties and Safety of A Lactiplantibacillus plantarum Strain
摘要
Abstract
[Objective]This study aimed to isolate and screen a duck-origin probiotic strain,investigate its biological characteristics,and select candidate strains for meat duck production,providing reference for developing novel probiotic preparations.[Method]Bacterial strain was isolated and purified from duck cecal contents.Identification was performed through calcium dissolution zone assay,Gram staining,biochemical tests,and 16S rDNA sequencing.Growth characteristics,acid tolerance,bile salt tolerance,free amino acid production,and organic acid content were determined.Antimicrobial efficacy against Escherichia coli,Riemerella anatipestifer,Salmonella spp.,Staphylococcus aureus,Streptococcus suis,and Avibacterium paragallinarum were evaluated through inhibition rate and apoptosis rate assays.Scanning electron microscopy characterized antibacterial morphology.Duck-derived Lactiplantibacillus plantarum extracellular proteins were isolated by ultrafiltration,with antibacterial components analyzed by liquid chromatography-tandem mass spectrometry(LC-MS/MS).Safety assessment was conducted in meat ducks.[Result]A Gram-positive,non-spore-forming bacillus was isolated.and it was determined to be Lactobacillus genus through biochemical identification tests.Then,it was confirmed to be Lactiplantibacillus plantarum through 16S rDNA detection.Compared with Lactiplantibacillus plantarum from pickle(pickle-LP),this Lactiplantibacillus plantarum from duck(duck-LP)could quickly enter the logarithmic phase and plateau of growth,and had a better acid and bile salt resistance.It could produce more free amino acids,lactic acid,and acetic acid(P＜0.05).It had a significant inhibitory effect on Escherichia coli and Avibacterium paragallinarum at a 1∶1 addition ratio(P＜0.05).At a 1∶4 addition ratio,there was a significant inhibitory effect on the growth of Escherichia coli,Riemerella anatipestifer,and Salmonella spp.(P＜0.05).At a 1∶9 addition ratio,there was a significant inhibitory effect on Escherichia coli and Riemerella anatipestifer(P＜0.05).At a 1∶4 addition ratio,the apoptosis rates of Escherichia coli,Riemerella anatipestifer,Streptococcus suis,and Avibacterium paragallinarum were significantly increased(P＜0.05).Through LC-MS/MS,a total of 10 proteins were detected,including two antimicrobial components:Peptidoglycan-binding protein and acetate kinase.The safety test on meat ducks showed that the bacterium was safe and had no toxic side effects on meat ducks.[Conclusion]This experiment isolated and screened a strain of duck-LP,which had excellent growth characteristics,acid/bile tolerance,amino acid/organic acid production,and broad-spectrum antimicrobial activity.Its antibacterial mechanism might be through synergistic effects of antimicrobial proteins and organic acids,disrupting cell wall/membrane integrity.The strain showed potential as a safe probiotic feed additive for meat ducks.关键词
植物乳杆菌/分离鉴定/生物学特性/安全性/肉鸭Key words
Lactiplantibacillus plantarum/isolation and identification/biological characteristics/safety/meat ducks分类
农业科技引用本文复制引用
代林,怀志芳,王宝维,张名爱,凡文磊,张晶,王秉翰,齐国峰..1株植物乳杆菌的分离鉴定、生物学特性和安全性研究[J].中国畜牧兽医,2025,52(7):3070-3083,14.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-42-14) （CARS-42-14）
2022年度山东省重点研发计划(2022LZGC014) （2022LZGC014）
