中国药房2025，Vol.36Issue(14)：1717-1721,5.DOI:10.6039/j.issn.1001-0408.2025.14.05
麻黄-桂枝药对对哮喘寒饮蕴肺证大鼠呼吸功能和气道炎症的影响及机制研究
Effects of Ephedra-Cinnamomum couplet medicinals on respiratory function and airway inflammation in rats with asthma of cold-fluid retention in lung syndrome and its mechanism
摘要
Abstract
OBJECTIVE To investigate the effects of Ephedra-Cinnamomum couplet medicinals on respiratory function and airway inflammation in rats with asthma of cold-fluid retention in lung syndrome and its mechanism.METHODS Sixty Wistar rats were randomly divided into blank group,model group,dexamethasone group[1 mg/(kg·d)],and Ephedra-Cinnamomum low-,medium-,high-dose groups[0.234,0.936,1.872 g/(kg·d)],with 10 rats in each group.The model and treatment groups were sensitized by intraperitoneal injection of antigen solution(ovalbumin 100 mg+aluminum hydroxide 100 mg)and challenged with 1%ovalbumin nebulization,along with exposure to a cold environment and ingestion of cold water,to establish the asthma model with cold-fluid retention in lung syndrome.From day 2,rats received corresponding drugs or normal saline intragastrically,once a day,for 21 consecutive days.The general behavioral changes in each group of rats were observed during the experimental process.The lung function parameters[peak expiratory flow(PEF),airway resistance(Raw),functional residual capacity(FRC),expiratory flow at 50%of forced vital capacity(EF50%)]were measured before modeling and after the last medication as well as serum contents of interleukin-6(IL-6),IL-13,tumor necrosis factor-α(TNF-α)and interferon-gamma(IFN-γ)after the last medication were determined;the histopathological morphological changes in the lung tissues of rats were also observed;mRNA expressions of IL-6,IL-3,TNF-α,IFN-γ,thymic stromal lymphopoietin(TSLP),and Toll-like receptor 4(TLR4),as well as protein expressions of TSLP and TLR4 were determined in lung tissue.RESULTS Compared with model group,the lung tissue damage of rats was relieved significantly;Raw,FRC,the contents and mRNA expression levels of IL-6,IL-13 and TNF-α,as well as the mRNA and protein expressions of TSLP and TLR4 were significantly decreased(P＜0.05),while the contents and mRNA expressions of PEF,EF50%and IFN-γ were significantly increased in the dexamethasone group and Ephedra-Cinnamomum medium-and high-dose groups(P＜0.05).Moreover,only a few rats in the two groups exhibited typical symptoms of asthma.CONCLUSIONS Ephedra-Cinnamomum couplet medicinals improve respiratory function and ameliorate airway inflammation in asthma rats with cold-fluid retention in lung syndrome,the mechanism of which may be associated with inhibiting TSLP/TLR4 signaling pathway and modulating Th1/Th2 imbalance.关键词
麻黄-桂枝药对/支气管哮喘/寒饮蕴肺证/胸腺基质淋巴细胞生成素/Toll样受体4Key words
Ephedra-Cinnamomum couplet medicinals/bronchial asthma/cold-fluid retention in lung syndrome/thymic stromal lymphopoietin/Toll-like receptor 4分类
医药卫生引用本文复制引用
赵明哲,王彬,赵晓旻,孙羽扬,颜培正..麻黄-桂枝药对对哮喘寒饮蕴肺证大鼠呼吸功能和气道炎症的影响及机制研究[J].中国药房,2025,36(14):1717-1721,5.基金项目
国家自然科学基金青年科学基金项目(No.82004233) （No.82004233）
山东省中医药科技项目(No.MR20241737,No.Q-2023122) （No.MR20241737,No.Q-2023122）
山东省医药卫生科技发展计划项目(No.202203020866) （No.202203020866）
