中国药房2025，Vol.36Issue(14)：1743-1748,6.DOI:10.6039/j.issn.1001-0408.2025.14.09
指纹图谱结合药效成分含量综合评价银丹活血通瘀颗粒质量
Comprehensive evaluation of the quality of Yindan huoxue tongyu granules based on fingerprint combined with content of pharmacodynamic components
摘要
Abstract
OBJECTIVE To evaluate the quality of Yindan huoxue tongyu granules.METHODS Taking high performance liquid chromatography with ultraviolet and evaporative light scattering detection as method,the fingerprint of 15 batches of Yindan huoxue tongyu granules was established,and similarity evaluation was performed by Similarity Evaluation System of Chromatographic Fingerprint of TCM(2012 edition)to determine common peaks.The common peaks were identified by comparing with reference substance chromatograms and single decoction piece chromatograms.Network pharmacology was used to screen out core targets and pathways of identified components,construct a"component-target-pathway"network diagram,and predict the pharmacodynamic components of Yindan huoxue tongyu granules,and the content determination of these components was carried out by the same method.RESULTS HPLC fingerprints of 15 batches of Yindan huoxue tongyu granules were characterized with 40 common peaks,and 17 components including salvianolic acid B,astragaloside Ⅳ,notoginsenoside R1,and ginkgolide A were identified.Network pharmacology predicted that 17 components mainly acted on 97 core targets and 137 pathways to exert their pharmacological effect.Average contents of 13 bioactive components in 15 batches of samples were 0.126 8,0.232 0,0.073 8,0.353 2,3.620 2,0.191 0,0.333 3,0.317 4,0.785 0,0.538 2,0.460 0,2.475 1 and 0.347 7 mg/g,including calycosin-7-O-β-D-glucoside,rosmarinic acid,formononetin,lithospermic acid,salvianolic acid B,ononin,ginsenoside Rb1,ginsenoside Rd,ginkgolide C,ginkgolide A,ginkgolide B,notoginsenoside R1,and astragaloside Ⅳ.CONCLUSIONS The established fingerprint of Yindan huoxue tongyu granules can reflect the overall characteristics of the preparation.The content determination method for its pharmacodynamic components,developed in combination with network pharmacology,is accurate,reliable,and exhibits good repeatability,making it suitable for evaluating the quality of Yindan huoxue tongyu granules.关键词
银丹活血通瘀颗粒/指纹图谱/含量测定/网络药理学/药效成分/质量评价Key words
Yindan huoxue tongyu granules/fingerprint/content determination/network pharmacology/pharmacodynamic components/quality evaluation分类
医药卫生引用本文复制引用
白淑贤,王单单,吴作敏,于晓涛,董敬远,李叶宁..指纹图谱结合药效成分含量综合评价银丹活血通瘀颗粒质量[J].中国药房,2025,36(14):1743-1748,6.基金项目
河南省科技攻关项目(No.242102310544) （No.242102310544）
河南省中医药科学研究专项课题(No.2023ZY3032) （No.2023ZY3032）
漯河市重大科技创新专项(揭榜挂帅)项目(No.漯科[2022]45号) （揭榜挂帅）
