中国药房2025，Vol.36Issue(14)：1749-1754,6.DOI:10.6039/j.issn.1001-0408.2025.14.10
补肾宁神膏的指纹图谱建立、含量测定及体外抗氧化作用的谱效关系研究
Study on fingerprint establishment,content determination,and spectrum-effect relationship of in vitro antioxidant activity for Bushen ningshen ointment
摘要
Abstract
OBJECTIVE To establish the fingerprint of Bushen ningshen ointment,determine the contents of its major constituents,and investigate its in vitro antioxidant activity.METHODS High performance liquid chromatography(HPLC)fingerprints of 10 batches of Bushen ningshen ointment were established.Similarity evaluation and identification of common peaks were subsequently performed.The contents of 10 components such as salidroside were determined using the same HPLC method.Using the scavenging rates against 2,2′-azino bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals,as well as ferric ion reducing antioxidant power(FRAP)as indicators,the anti-oxidant activity of the ointment was evaluated;grey relational analysis and partial least squares regression were conducted using SIMCA 14.1 software to establish the spectrum-effect relationship.RESULTS The fingerprint chromatogram of 10 batches of Bushen ningshen ointment contained 24 common peaks,with similarity values all exceeding 0.96.Eleven peaks were identified as adenosine(peak 1),salidroside(peak 4),morroniside(peak 6),catechin(peak 7),paeoniflorin(peak 10),spinosin(peak 11),ferulic acid(peak 12),isoquercitrin(peak 13),verbascoside(peak 14),paeonol(peak 23),and emodin(peak 24).Content determination results showed that the average contents of salidroside,morroniside,catechin,paeoniflorin,spinosin,ferulic acid,isoquercitrin,verbascoside,paeonol,and emodin were 0.725,1.962,0.214,3.395,0.124,0.107,0.286,0.019,0.034 and 0.067 mg/g,respectively.The antioxidant potency composite index(APC)for the 10 batches ranged from 85.08%to 96.35%.Spectrum-effect relationship analysis indicated that all 24 common peaks were positively correlated with the antioxidant capacity.Seventeen peaks had variable importance in projection values＞1,specitically peaks 2,5,6,7,9,10,13-21,23,and 24.CONCLUSIONS This study successfully established the HPLC fingerprint and content determination method for Bushen ningshen ointment.The compounds represented by the 17 common peaks such as morroniside may be the active components contributing to its antioxidant effects.关键词
补肾宁神膏/指纹图谱/谱效关系/抗氧化活性/含量测定Key words
Bushen ningshen ointment/fingerprint/spectrum-effect relationship/antioxidant activity/content determination分类
医药卫生引用本文复制引用
王丽,徐文华,杨洋,辛灵怡,张蓁怡,杨光义..补肾宁神膏的指纹图谱建立、含量测定及体外抗氧化作用的谱效关系研究[J].中国药房,2025,36(14):1749-1754,6.基金项目
广东省中医药局中医药科研项目(No.20251335) （No.20251335）
深圳市"医疗卫生三名工程"项目(No.SZZYSM202106009) （No.SZZYSM202106009）
深圳市宝安区中医药临床研究专项(No.2023ZYYLCZX-14) （No.2023ZYYLCZX-14）
深圳市中西医结合医院院内科研课题(No.YJ-2023-108) （No.YJ-2023-108）
