中国医科大学学报2025，Vol.54Issue(7)：577-582,6.DOI:10.12007/j.issn.0258-4646.2025.07.001
经颅磁刺激与人脐带间充质干细胞联合治疗通过抑制铁死亡促进脊髓损伤修复
Transcranial magnetic stimulation combined with human umbilical cord mesenchymal stem cells promotes spinal cord injury repair through inhibiting ferroptosis
摘要
Abstract
Objective To investigate the effects of transcranial magnetic stimulation(TMS)combined with human umbilical cord mesen-chymal stem cells(hUC-MSCs)on ferroptosis following spinal cord injury(SCI)in rats.Methods Allen percussion was used to estab-lish the SCI model.A total of 60 SD rats were randomly divided into sham,SCI,TMS,hUC-MSC,and TMS+hUC-MSC groups,with 12 rats in each group.Motor function was evaluated using the Basso-Beattie-Bresnahan(BBB)score.Spinal cord tissues were sampled and stained with hematoxylin and eosin(HE)as well as Nissl to observe tissue damage as well as the changes in neurons and Nissl bodies,respectively.The colorimetric method was used to detect the contents of ferrous ions(Fe2+)and reduced glutathione(GSH).Transmission electron microscopy was applied to observe the ultrastructure of the mitochondria.Western blotting was performed to detect the expres-sion levels of SLC7A11,GPX4,and ACSL4.Results The SCI group had lower BBB scores,higher Fe2+and ACSL4 protein expression levels,and lower GSH,SLC7A11,and GPX4 protein expression levels than the sham group(P<0.05).The mitochondrial cristae reduced with membrane shrinkage,neuronal damage was severe,and Nissl bodies were absent in the SCI group.The TMS,hUC-MSC,and TMS+hUC-MSC groups had higher BBB scores,lower Fe2+and ACSL4 protein expression levels,and higher GSH,SLC7A11,and GPX4 protein expression levels than the SCI group(P<0.05).The mitochondrial cristae increased with an intact membrane structure,the pathological damage was attenuated,neuronal morphology was restored,and Nissl bodies were clearly visible in TMS,hUC-MSC,and TMS+hUC-MSC groups.Conclusion TMS combined with hUC-MSC inhibits ferroptosis through activating the SLC7A11/GSH/GPX4 pathway,alleviates secondary injury after SCI,and promotes functional recovery and neural remodeling in rats.关键词
脊髓损伤/铁死亡/经颅磁刺激/人脐带间充质干细胞Key words
spinal cord injury/ferroptosis/transcranial magnetic stimulation/human umbilical cord mesenchymal stem cell分类
医药卫生引用本文复制引用
华祚玉,王雅诗,孙师,张立新..经颅磁刺激与人脐带间充质干细胞联合治疗通过抑制铁死亡促进脊髓损伤修复[J].中国医科大学学报,2025,54(7):577-582,6.基金项目
国家自然科学基金(81101462) （81101462）
辽宁省自然科学基金(2022-YGJC-56) （2022-YGJC-56）
辽宁省重点研发项目(2024JH2/102500021) （2024JH2/102500021）
