中国药理学与毒理学杂志2025，Vol.39Issue(7)：511-517,7.DOI:10.3867/j.issn.1000-3002.2025.08213
白藜芦醇对芥子气致小鼠急性肺损伤的改善作用及可能机制
Improvement of sulfur mustard-induced acute lung injury by resveratrol in mice and the potential mechanism
摘要
Abstract
OBJECTIVE To study how resveratrol(Res)mitigates acute lung injury(ALI)in mice induced by sulfur mustard(SM)and potential mechanism.METHODS Male C57BL/6N mice were randomly divided into the control group(50 μL physiological saline via nebulization),SM group(SM 5 mg·kg-1 via nebulization),SM+Res 10 or 20 mg·kg-1 groups(1 h after administration of SM 5 mg·kg-1,Res 10 or 20 mg·kg-1 was administered via nebulization).Mice in each group were weighed 0,24,48 and 72 h after SM administration.Mice were sacrificed 72 h after SM administration,and lung tissues were collected,weighed for wet and dry weights,and the wet/dry ratio was calculated.HE staining was employed to detect the pathological changes of lung tissues while real-time quantitative PCR(RT-qPCR)was used to detect the mRNA expression levels of interleukin 6(IL-6)and IL-1β.Transcriptomic changes of the SM group and the SM+Res 20 mg·kg-1 group were detected with the next-generation sequencing technology.RT-qPCR was used to detect the mRNA expression changes of adenosine 5'-monophos-phate-activated protein kinase(AMPK)and silence information regulator 1(SIRT1)in lung tissues.RESULTS 72 h after SM administration,the body mass of mice in the SM group was significantly decreased compared with normal control group,while the wet/dry ratio of the lung was significantly increased,so were the mRNA expression levels of IL-6 and IL-1β in lung tissues were also significantly increased.Pathological changes of lung tissues included alveolar cavity atrophy,marked parenchymal tissues,and diffuse infiltration of local inflammatory cells.Compared with the SM group,the body mass of mice in the SM+Res 10 and 20 mg·kg-1 groups significantly increased,the wet/dry ratio of lung tissues was significantly reduced,and expressions of IL-6 and IL-1β mRNA were significantly decreased in SM+Res 10 and 20 mg·kg-1 groups.Compared with the normal control group,the mRNA expression levels of AMPK and SIRT1 in lung tissues of the SM group were significantly decreased.Compared with the SM group,the mRNA expression levels of AMPK in lung tissues of the SM+Res 10 and 20 mg·kg-1 groups were significantly increased while the mRNA expression level of SIRT1 was significantly decreased.CONCLUSION Res can mitigate ALI in mice induced by SM,and the mechanism may be related to the inhibition of cell apoptosis by regulating the AMPK/SIRT1 signaling pathway.关键词
芥子气/急性肺损伤/转录组/白藜芦醇/AMP依赖的蛋白激酶/沉默信息调节因子Key words
sulfur mustard/acute lung injury/transcriptome/resveratrol/adenosine 5'-monophos-phate-activated protein kinase/silence information regulator 1分类
医药卫生引用本文复制引用
黄丽娟,杜冰,徐子瑛,袁静..白藜芦醇对芥子气致小鼠急性肺损伤的改善作用及可能机制[J].中国药理学与毒理学杂志,2025,39(7):511-517,7.基金项目
国家自然科学基金(82372270) （82372270）
国家自然科学基金(82302540) （82302540）
北京市博士后基金 （）
北京市自然科学基金(7244288) National Natural Science Foundation of China(82372270) （7244288）
National Natural Science Foundation of China(82302540) （82302540）
Postdactoral Research Fund of Beijing （）
and Natural Science Foundation of Beijing Municipality(7244288) （7244288）
