中国药理学与毒理学杂志2025，Vol.39Issue(7)：518-526,9.DOI:10.3867/j.issn.1000-3002.2025.08366
丹酚酸A对体外活化后的中性粒细胞不同功能的调节作用
Effects of salvianolic acid A on functions of neutrophils after activation in vitro
摘要
Abstract
OBJECTIVE To investigate the effect of salvianolic acid A(SAA)on functions of neutro-phils after activation in vitro.METHODS Rat neutrophils were extracted and activated by lipopolysac-charide(LPS)at 0.3,1,3 mg·L-1,and the number of adherent neutrophils and myeloperoxidase(MPO)activity were detected to determine the concentration of LPS.Neutrophils were divided into the control,model,model+4-aminobenzohydrazide(ABH)20 μmol·L-1,and model+SAA 1,3 and 10 μmol·L-1 groups.LPS was stimulated with 3 mg·L-1 for 30 min,and the neutrophil adhesion rate was detected by immunofluorescence after 1 h of drug incubation.After 2 h of drug incubation,phagocytosis of neutro-phils was detected by immunofluorescence and fluorescein isothiocyanate-immunoglobulin G.After 3 h of drug incubation,the neutrophil adhesion rate to endothelial cells was detected by colorimetric assay.Intracellular MPO activity and hypochlorous acid(HOCl)production were investigated by colorimetric assay in response to the degranulation function.Intracellular reactive oxygen species(ROS)levels were detected by probe assay,and mitochondrial membrane potential by JC-1 assay.The levels of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione(GSH)and total antioxidant capacity(T-AOC)were measured to reflect oxidation function of neutrophils.RESULTS LPS increased the number of adherent cells and MPO activity in a concentration-dependent manner,with 3 mg·L-1 of LPS showing the most significant effect,which was used for subsequent experiments.Compared with the control group,LPS-activated neutrophil adhesion and phagocytosis were significantly enhanced.MPO activity and HOCl production significantly increased.The levels of ROS and MDA in LPS-activated neutrophils were significantly increased while the mitochondrial membrane potential and the levels of SOD,GSH,T-AOC were significantly decreased,indicating that the oxidative stress ability was enhanced.Compared with the model group,SAA dose-dependently inhibited LPS-induced adhesion,phagocytosis,degranu-lation,and ROS generation of neutrophils,with significant effects at medium and high doses.CONCLU-SION SAA can inhibit different functions of neutrophils after activation,which may be a potential drug for targeting neutrophil function regulation.关键词
丹酚酸A/中性粒细胞/氧化应激/黏附/吞噬/脱颗粒/ROS生成Key words
salvianolic acid A/neutrophils/oxidative stress/adhesion/phagocytosis/degranula-tion/reactive oxygen species production分类
医药卫生引用本文复制引用
王珑睿,赵子源,姜胤如,李朝唯,孙闻婧,杜冠华,孔令雷..丹酚酸A对体外活化后的中性粒细胞不同功能的调节作用[J].中国药理学与毒理学杂志,2025,39(7):518-526,9.基金项目
国家自然科学基金(82474112) （82474112）
中国医学科学院医学与健康科技创新工程(2022-I2M-1-015) National Natural Science Foundation of China(82474112) （2022-I2M-1-015）
and CAMS Innovation Fund for Medical Sciences(2022-I2M-1-015) （2022-I2M-1-015）
