液质联用法检测延胡索饮片中5种违规添加着色剂OA

Objective:To establish a method for the determination of five yellow pigments(Lemon yellow,Sunset yellow,Orange Ⅱ,Auramine O,Acid yellow 36)added into corydalis rhizoma pieces by HPLC-MS/MS.Methods:The analysis was performed with Aglient triple quadrupole mass spectrometer.Electrospray ion(ESI)was adopted as the ion source with positive and negative ion scanning mode(MRM).The Aglient Eclipse Plus C18(2.1 mm × 100 mm,2.2 μm)column was used with acetonitrile-0.02 mol·L-1 ammonium acetate solution as the mobile phase.The flow rate was 0.3 mL·min-1,the columm temperature was 40 ℃.Results:The result showed good linearity in the ranges of 0.40-20.00 μg·mL-1 for lemon yellow and sunset yellow,0.05-2.50 μg·mL-1 for orange Ⅱ,auramine O and acid yellow 36.The limits of detection were 0.1 μg·mL-1 and 0.02 μg·mL-1.The average recovery of five yellow pigments was in the range of 89.6％-97.5％.None of the above five yellow pigments were detected in 10 batches of corydalis rhizoma pieces.Conclusion:The method is convenient,reliable and accurate,and can be used for the detection of yellow pigments in corydalis rhizoma pieces.It's necessary for the quality control of corydalis rhizoma pieces.