中国药物评价2025，Vol.42Issue(3)：190-195,6.
超滤结合UHPLC-UV法测定川楝素在SD大鼠和新西兰兔中的血浆蛋白结合率
Determination of Plasma Protein Binding Rate of Toosendanin in SD Rats and New Zealand Rabbits by Ultrafiltration Combined with UHPLC-UV
梅赞 1于潜 1殷华锋 2梁基琳1
作者信息
- 1. 吉安市食品药品检验检测中心,江西吉安 343000
- 2. 江西青峰药业有限公司,江西赣州 341000
- 折叠
摘要
Abstract
Objective:To establish a method for the determination of plasma protein binding of toosendanin.Methods:An ultrafiltra-tion tube with a cut-off value of 10 K was used to separate the SD rats and New Zealand rabbits plasma bound and free toosendanin,and the separation was carried out on a Waters UPLC ® BEH C18(2.1 mm × 100 mm,1.7 μm)column with an isocratic elution of acetoni-trile-0.1％phosphoric acid(31∶69)as the mobile phase,and the flow rate was 0.3 mL·min-1 at 25 ℃.Results:The linear range was 1-50 μg·mL-1,the LLOQ was 0.5 μg·mL-1,and the results of the methodological validation were within acceptable limits.The plasma protein binding rates of 5,10 and 20 μg·mL-1 toosendaninin SD rats were(77.01±3.45)％,(81.30±1.34)％and(83.89±2.78)％,respectively.The plasma protein binding rates at 3 concentrations of toosendaninin in New Zealand rabbit plasma was(64.09±4.66)％,(71.55±3.16)％,and(73.48±3.28)％,respectively.Conclusion:In vitro plasma protein binding of toosenda-nin did not show a concentration dependence with increasing plasma concentration of toosendanin,the binding rate was not affected by spe-cies,and there was almost no difference in protein binding between the plasma of SD rats and New Zealand rabbits.This research work can lay the foundation for further development and utilization of the medicinal value of toosendanin.关键词
川楝素/超高效液相色谱/血浆蛋白结合率/SD大鼠/新西兰兔Key words
Toosendanin/ultra High performance liquid chromatography(UHPLC)/Plasma protein binding rate/SD rats/New Zea-land rabbit分类
医药卫生引用本文复制引用
梅赞,于潜,殷华锋,梁基琳..超滤结合UHPLC-UV法测定川楝素在SD大鼠和新西兰兔中的血浆蛋白结合率[J].中国药物评价,2025,42(3):190-195,6.
