中国医学科学院学报2025，Vol.47Issue(3)：343-353,11.DOI:10.3881/j.issn.1000-503X.16238
SORT1在胃癌组织中的表达及其对胃癌细胞生物学的影响
Expression of SORT1 in Gastric Cancer Tissue and Its Effect on Gastric Cancer Cell Biology
摘要
Abstract
Objective To investigate the expression of SORT1 in the gastric cancer tissue and analyze its relationship with clinical prognosis of patients as well as the pathways and mechanisms involved in gastric cancer progression.Methods The Gene Expression Profiling Interaction Analysis database,Western blot,and immunohistochemistry were employed to predict and analyze the expression of SORT1 in the gastric cancer and the adjacent tissue.The clinical case information of 109 patients who underwent radical surgery for gastric cancer in the First Affiliated Hospital of Bengbu Medical University from April 2015 to April 2017 was collected to analyze the relationship of SORT1 with the clinicopathological parameters and prognosis of the patients.Cell proliferation was detected by the CCK-8 assay and colony formation assay,while cell migration and invasion were assessed by the scratch assay and Transwell assay,respectively.Western blot was employed to determine the expression of pro-teins related to epithelial-mesenchymal transition(EMT)in gastric cancer cells,followed by further analysis on molecular mechanism through which SORT1 regulates EMT in gastric cancer cells.Results Western blot and im-munocytochemistry results showed that SORT1 was highly expressed in the gastric cancer tissue(P=0.003,P＜0.001),which was positively correlated with malignant progression of tumors(all P＜0.05).The Kaplan-Meier survival analysis revealed shortened postoperative survival periods for the patients with high expression of SORT1(P＜0.001).The Cox regression model indicated that SORT1 expression was an independent risk fac-tor affecting the 5-year survival rate after surgery for gastric cancer patients(P＜0.001).Up-regulation of SORT1 expression promoted the proliferation,migration,invasion,and EMT of gastric cancer cells(all P＜0.05),while down-regulation of SORT1 showed the opposite effects(all P＜0.05).Western blot results showed that high expression of SORT1 promoted the expression of β-catenin,cyclin D1,and c-Myc(all P＜0.05).Moreover,in vitro use of the Wnt/β-catenin pathway inhibitor(XAV939)effectively suppressed the EMT enhancement caused by high expression of SORT1 in gastric cancer cells(all P＜0.05).Conclusions SORT1 is highly expressed in gastric cancer and affects patients' postoperative survival periods.It is involved in the proliferation,migration,and invasion of gastric cancer cells and may promote the EMT of gastric cancer cells by activating the Wnt/β-catenin pathway.关键词
胃癌/SORT1/预后/上皮间质转化,Wnt/β-连环蛋白通路Key words
gastric cancer/SORT1/prognosis/epithelial-mesenchymal transition/Wnt/β-catenin pathway分类
医药卫生引用本文复制引用
肖林雨,段婷,夏勇生,陈悦,闫兴洲,胡建国..SORT1在胃癌组织中的表达及其对胃癌细胞生物学的影响[J].中国医学科学院学报,2025,47(3):343-353,11.基金项目
癌症转化医学安徽省重点实验室开放课题(KFDX202202)、安徽省新时代育人质量工程项目(研究生教育)(2023xscx123)和蚌埠医科大学研究生科研创新计划(Byycxz23045) （KFDX202202）
