中国药业2025，Vol.34Issue(14)：81-85,5.DOI:10.3969/j.issn.1006-4931.2025.14.018
北沙参水溶性成分高效液相色谱特征图谱建立及核苷类成分含量测定
Establishment of HPLC Characteristic Chromatogram of Water-Soluble Components and Content Determination of Nucleosides in Glehniae Radix
摘要
Abstract
Objective To establish the HPLC characteristic chromatogram of Water-soluble components in Glehniae Radix,and to detecte the contents of 3 nucleoside components in medicinal materials.Methods The chromatography column was the Waters XBridge® C18 column(250 mm×46 mm,5 μm),the mobile phase was water and methanol(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 260 nm,the column temperature was 30℃,and the injection volume was 10 μL.The similarity evaluation was performed using the Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System software(2012 version)to determine common peaks and conduct cluster analysis.The contents of 3 nucleoside components were determined by HPLC.ResuLts A total of 6 common peaks were marked in the characteristic chromatograms of 15 batches of samples,and 3 common components were identified,and 3 of them were characterized as uridine,guanosine,and adenosine.15 batches of Samples could be clustered into two categories.The similarity of 15 batches of samples was higher than 0.9.The linear ranges of the above 3 nucleoside components were 1.11-22.19 μg/mL(R2=0.999 9)、0.98-14.69 μg/mL(R2=0.999 8)、2.01-20.12 μg/mL(R2=1.000 0).The limit of detection was 0.20,0.04,0.20 mg/mL,the limit of quantitation was 0.40,0.08,0.40 mg/mL.The RSDs of precision,stability and repeatability tests were all less than 2.0%.The average recovery rates were 93.49%,97.44%and 98.35%,with RSDs of 1.07%,1.14%and 0.58%(n=6),respectively.Conclusion The established characteristic chromatogram of water-soluble components and the conten determination method for 3 nucleosides in Glehniae Radix is satisfactory stability,reliability,and reproducibility,which can be applied for quality evaluation and control of Glehniae Radix.关键词
北沙参/水溶性成分/特征图谱/含量测定/高效液相色谱法Key words
Glehniae Radix/Water-soluble components/characteristic chromatogram/content determination/High-performance liquid chromatography分类
医药卫生引用本文复制引用
朱伟堃,赵蕊蕊,赵丹彤,刘延娟,李瑞英..北沙参水溶性成分高效液相色谱特征图谱建立及核苷类成分含量测定[J].中国药业,2025,34(14):81-85,5.基金项目
山东省中药标准物质研究项目[2022-SDY0023]. （）
