中华中医药杂志2025，Vol.40Issue(5)：2117-2122,6.
针灸对克罗恩病大鼠结肠DNA甲基化图谱特征的影响
Effects of acupuncture and moxibustion on DNA methylation profiles in colonic tissue of Crohn disease rats
摘要
Abstract
Objective:To explore the effects of acupuncture and moxibustion on the DNA methylation profile of colonic tissues in Crohn disease(CD)rats from a whole-genome perspective.Methods:SD rats were randomly divided into a normal group,model group,herb-partitioned moxibustion group,and electroacupuncture group.CD models were established using TNBS enemas,and interventions were carried out at the Tianshu(bilateral)and Qihai acupoints.Disease activity index(DAI)scores and colonic histopathology and scores were used to evaluate the effects of interventions.Whole genome bisulfite sequencing(WGBS)and bioinformatics were employed to identify differentially methylated region(DMR)in promoter regions,with RT-qPCR used for transcriptional validation.Results:The model group showed partial colonic glandular loss,fissure-like ulcerations,and high inflammatory infiltration,with significantly increased DAI and histopathology scores compared to the normal group(P＜0.01,P＜0.05).And hypomethylation as the dominant DMR pattern.Both herbal-partitioned moxibustion group and electroacupuncture group showed colonic mucosal epithelial repair,reduced infammation,and signifcantly lower DAI and histopathology scores compared to the model group(P＜0.05),with hypermethylation as the major DMR feature.And both herbal-partitioned moxibustion group and electroacupuncture group significantly downregulated the mRNA expression of Hspa5,Ctnnb1,Cox6b1,and Krt8 in the colon compared to the model group(P＜0.01,P＜0.05).Conclusion:Herb-partitioned moxibustion and electroacupuncture modulate DNA methylation in CD rats,increasing the DNA methylation in the promoter regions of colonic Hspa5,Ctnnb1,Cox6b1 and Krt8,which may serve as an epigenetic mechanism in acupuncture-based therapy for CD.关键词
克罗恩病/表观遗传学/DNA甲基化/隔药灸/电针/机制/差异甲基化区域Key words
Crohn disease(CD)/Epigenetics/DNA methylation/Herb-partitioned moxibustion/Electroacupuncture/Mechanism/Differentially methylated region(DMR)引用本文复制引用
徐宏潇,祁琴,吴焕淦,黄艳,朱璐,吴璐一,邓指辉,李峰,陆嫄,郑诗雨,李国娜,马喆..针灸对克罗恩病大鼠结肠DNA甲基化图谱特征的影响[J].中华中医药杂志,2025,40(5):2117-2122,6.基金项目
国家自然科学基金项目(No.82074546,No.82422074,No.82205262),上海市科学技术委员会"扬帆计划"(No.22YF1444100) （No.82074546,No.82422074,No.82205262）
