中国肿瘤生物治疗杂志2025，Vol.32Issue(7)：698-705,8.DOI:10.3872/j.issn.1007-385x.2025.07.004
2',4'-二羟基查尔酮通过调控miR-7-5p诱导自噬抑制结直肠癌细胞增殖和迁移
2',4'-dihydroxychalcone inhibits the proliferation and migration of colorectal cancer cells by regulating miR-7-5p-induced autophagy
摘要
Abstract
Objective:To investigate the molecular mechanism by which 2',4'-dihydroxychalcone(D2)inhibits proliferation,migration,and epithelial-mesenchymal transition(EMT)in colorectal cancer cells through miR-7-5p-mediated autophagy.Methods:Human colorectal cancer cell lines HCT-15 and SW620 were treated with D2 at concentrations of 0,12.5,25,and 50 µmol/L.Cell proliferation and clonogenic capacity were evaluated using MTT and colony formation assays.Cell migration was assessed by wound healing and Transwell assays.WB assay was used to detect the expression of EMT-related proteins,autophagy-related proteins,and key components of the PI3K/AKT/mTOR pathway.Autophagosome formation was visualized by immunofluorescence staining.TCGA database and KEGG pathway analyses were performed to evaluate miR-7-5p expression and its association with colorectal cancer.RT-qPCR was used to quantify miR-7-5p expression,and lentiviral transduction was employed to establish stable miR-7-5p knockdown or overexpression cell lines.Results:D2 significantly inhibited colorectal cancer cell proliferation,migration,and EMT(P<0.05 or P<0.01).TCGA and KEGG analyses revealed that miR-7-5p expression was downregulated in colorectal cancer and closely associated with disease progression.D2 treatment(12.5,25,and 50 µmol/L)significantly upregulated miR-7-5p expression in HCT-15 and SW620 cells(P<0.01).At 25 µmol/L,D2 increased the expression of autophagy-related proteins(LC3 and p-ULK1)and inhibited the PI3K/AKT/mTOR signaling pathway(P<0.05),accompanied by increased autophagosome formation(P<0.01).In miR-7-5p-knockdown cells treated with D2,the levels of LC3 and p-ULK1 were significantly reduced compared to D2-only treated cells(P<0.05 or P<0.01).Conclusion:D2 upregulates miR-7-5p to induce autophagy,thereby inhibiting colorectal cancer cell proliferation,migration,and EMT,possibly through suppression of the PI3K/AKT/mTOR signaling pathway.关键词
结直肠癌/2',4'-二羟基查尔酮/自噬/细胞增殖/上皮间质转化Key words
colorectal cancer/2',4'-dihydroxychalcone(D2)/autophagy/cell proliferation/epithelial-mesenchymal transition(EMT)分类
医药卫生引用本文复制引用
苏朝霞,李文静,王南苗,陈丹,韩有艳,毕遥,王彤,安仁波,朴英实,任香善..2',4'-二羟基查尔酮通过调控miR-7-5p诱导自噬抑制结直肠癌细胞增殖和迁移[J].中国肿瘤生物治疗杂志,2025,32(7):698-705,8.基金项目
国家自然科学基金项目(No.82260678) （No.82260678）
吉林省科技厅项目(No.YDZJ202201ZYTS173) （No.YDZJ202201ZYTS173）
