中国肿瘤生物治疗杂志2025，Vol.32Issue(7)：731-737,7.DOI:10.3872/j.issn.1007-385x.2025.07.008
lncRNA SOX2-OT调控miR-215-5p/NOB1通路抑制结直肠癌HCT-116细胞增殖和迁移
lncRNA SOX2-OT inhibits proliferation and migration of colorectal cancer HCT-116 cells via the miR-215-5p/NOB1 axis
摘要
Abstract
Objective:To investigate whether lncRNA SOX2-OT inhibits the proliferation and migration of colorectal cancer(CRC)HCT-116 cells by regulating the miR-215-5p/NIN/RPN12 binding protein 1 homolog(NOB1)signaling pathway.Methods:Cancerous and paired adjacent tissue samples from 29 CRC patients treated at Wuhan Jinyintan Hospital from June 2022 to May 2024 were collected,along with CRC cell lines(SW480,HCT-116,HP116,and LoVo)and normal human colon epithelial HCoApiC cells.The mRNA expression levels of SOX2-OT,miR-215-5p,and NOB1 in CRC tissues and cells were measured using qPCR method.HCT-116 cells were transfected with SOX2-OT knockdown or overexpression plasmids and corresponding negative control plasmids using RNA interference technology,dividing the cells into control group,si-NC group,si-SOX2-OT group,si-SOX2-OT+inhibitor(Inh)NC group,si-SOX2-OT+miR-215-5p Inh group,si-SOX2-OT+oe-NC group,and si-SOX2-OT+oe-NOB1 group.The mRNA expression levels of SOX2-OT,miR-215-5p,and NOB1 in each group of cells were detected using qPCR method.MTT assay,scratch wound healing assay,Transwell chamber assay,and flow cytometry were used to measure cell proliferation,migration,invasion,and apoptosis,respectively.Western blot was applied to detect protein expression levels of E-cadherin,N-cadherin,vimentin,Bcl-2,BAX,PCNA,MMP-9,and NOB1.The targeting relationship between miR-215-5p and SOX2-OT or NOB1 was validated using dual-luciferase reporter gene assays.Results:SOX2-OT and NOB1 mRNA were significantly upregulated,while miR-215-5p was downregulated in both CRC tissues and cells(all P<0.05).In HCT-116 cells with SOX2-OT knockdown,the expression of SOX2-OT and NOB1 mRNA,cell proliferation,wound healing rate,invasive cell number,and protein levels of N-cadherin,vimentin,Bcl-2,NOB1,PCNA,and MMP-9 were significantly reduced(all P<0.05),while miR-215-5p expression,apoptosis rate,and protein levels of E-cadherin and BAX were significantly increased(all P<0.05).Both miR-215-5p knockdown and NOB1 overexpression reversed the inhibitory effects of SOX2-OT knockdown on HCT-116 cells(both P<0.05).miR-215-5p was validated to target SOX2-OT and NOB1.Conclusion:SOX2-OT knockdown upregulates miR-215-5p expression and downregulates NOB1 expression,further inhibiting the proliferation,migration,and invasion of HCT-116 cells and promoting apoptosis.关键词
lncRNA SOX2-OT/miR-215-5p/NOB1轴/结直肠癌/HCT-116细胞/增殖/迁移/侵袭Key words
lncRNA SOX2-OT/miR-215-5p/NOB1 axis/colorectal cancer(CRC)/HCT-116 cell/proliferation/migration/invasion分类
医药卫生引用本文复制引用
刘丹,程海林,罗剑锋..lncRNA SOX2-OT调控miR-215-5p/NOB1通路抑制结直肠癌HCT-116细胞增殖和迁移[J].中国肿瘤生物治疗杂志,2025,32(7):731-737,7.基金项目
武汉市卫生健康委员会资助项目(No.WX17D19) （No.WX17D19）
