中国中西医结合影像学杂志2025，Vol.23Issue(4)：444-448,504,6.DOI:10.3969/j.issn.1672-0512.2025.04.008
电针干预改善大脑中动脉栓塞大鼠脑部缺血机制的研究
Electroacupuncture intervention on mechanism of cerebral ischemia in rats with middle cerebral artery occlusion(MCAO)
摘要
Abstract
Objective:To observe the effect of electroacupuncture on the expression of HtrA1 in astrocytes within the hippocampal tissue of rats with acute cerebral infarction.Methods:Twenty-four male SD rats were selected and randomly divided into the sham-operation,model,and electroacupuncture groups,with 8 rats in each group.The model and electroacupuncture groups underwent right middle cerebral artery occlusion(MCAO)modeling via the intraluminal filament occlusion method.The sham-operation group received identical surgical exposure without filament insertion.Twenty-four hours after successful modeling,rats in the electroacupuncture group received electroacupuncture treatment at Baihui(GV20)and Shenting(GV24)using dense-disperse waves with alternating frequencies of 2/15 Hz and a current intensity of 1.5-2 mA.Cerebral blood flow changes,astrocyte expression and activation status,HtrA1 mRNA levels,HtrA1 protein expression levels,and TNF-α,IL-1β,and IL-6 levels of three groups were measured and compared seven days after the operation.Results:Western blotting showed that HtrA1 expression in the model group was significantly increased compared to the sham-operation and electroacupuncture groups(both P<0.05).Quantitative real-time PCR(qRT-PCR)showed that HtrA1 mRNA expression level in the electroacupuncture group was significantly lower than that in the model group(P<0.05).ELISA showed that the model group had the elevated levels of TNF-α,IL-1β,and IL-6 compared to the sham-operation group(all P<0.05),while the electroacupuncture group had the reduced levels of TNF-α,IL-1β,and IL-6 compared to the model group(all P<0.05).Conclusions:Electroacupuncture can alleviate injury,improve neurological function,enhance cerebral blood flow,and suppress the inflammatory response,and the mechanism may be associated with the inhibition of HtrA1 protein expression and suppression of astrocyte polarization.关键词
电针/星形胶质细胞/脑缺血再灌注/脑梗死Key words
Electroacupuncture/Astrocytes/Cerebral ischemia reperfusion/Cerebral infarction引用本文复制引用
杨韵蓉,冯辉,管华宗..电针干预改善大脑中动脉栓塞大鼠脑部缺血机制的研究[J].中国中西医结合影像学杂志,2025,23(4):444-448,504,6.基金项目
上海市卫生健康委员会中医药科研项目青年引导类项目(2024QN011) （2024QN011）
徐汇区卫生健康系统尖峰学科建设资助项目(SHXHZDXK202301). （SHXHZDXK202301）
