中国中医急症2025，Vol.34Issue(7)：1148-1151,1174,5.DOI:10.3969/j.issn.1004-745X.2025.07.005
通督灸调节Keap1/Nrf2/ARE信号通路对急性脑缺血再灌注大鼠氧化应激损伤的影响
Effect of Tongdu Moxibustion on Oxidative Stress Injury in Acute Cerebral Ischemia-Reperfusion Rats by Regulating the Keap1/Nrf2/ARE Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of Tongdu moxibustion on oxidative stress injury in acute cere-bral ischemia-reperfusion(CIR)rats by regulating the Keap1/Nrf2/ARE signaling pathway.Methods:90 SD rats were divided into sham operation group,model group,model+Nrf2 inhibitor all-trans retinoic acid(ATRA)group(10 mg/kg),edaravone group(3 mg/kg),Tongdu moxibustion group,and Tongdu moxibustion+ATRA group(10 mg/kg).Neurological function was assessed using Longa scoring criteria,and brain infarct volume was measured by TTC staining.Pathological changes in the ischemic cortex were observed by HE staining.Superoxide dismutase(SOD)activity and malondialdehyde(MDA)content in serum were measured using the WST-8 colorimetric meth-od and thiobarbituric acid(TBA)method,respectively.Reactive oxygen species(ROS)levels in brain tissue were analyzed using the 2',7'-dichlorofluorescin diacetate(DCFH-DA)staining method.Protein expression levels of Keap1,Nrf2,HO-1,and NQO1 in the ischemic cortex were detected by Western blotting.Results:Compared with the model group,the Tongdu moxibustion group showed significantly reduced pathological changes in the ischemic cortex,decreased neurological deficit scores,infarct volume percentage,MDA content,and Keap 1 protein expres-sion,while increased SOD activity,nuclear Nrf2/Nrf2,HO-1,and NQO1 protein expression(0.05).After ATRA in-tervention,the therapeutic effects of Tongdu moxibustion on acute cerebral ischemia-reperfusion rats were weak-ened(P＜0.05).Conclusion:Tongdu moxibustion has a significant effect on reducing oxidative stress injury in acute cerebral ischemia-reperfusion rats,which may be related to the upregulation of the Keap1/Nrf2/ARE signal-ing pathway.关键词
脑缺血再灌注/艾灸/通督灸/Kelch样环氧氯丙烷相关蛋白1/核因子E2相关因子2/抗氧化反应元件信号通路/氧化应激/大鼠Key words
Cerebral ischemia-reperfusion/Moxibustion/Tongdu moxibustion/Keap1/Nrf2/ARE signaling path-way/Oxidative stress/Rats分类
医药卫生引用本文复制引用
陈雪妮,秦静,张向悦,秦杰,武扬,赵保东,张天生,张捷..通督灸调节Keap1/Nrf2/ARE信号通路对急性脑缺血再灌注大鼠氧化应激损伤的影响[J].中国中医急症,2025,34(7):1148-1151,1174,5.基金项目
张捷省级名中医传承工作室建设项目(SXSMZYGZS130) （SXSMZYGZS130）
山西中医药大学科技创新团队(培育)(2022TD2001) （培育）
山西省中医药管理局科研课题(2024ZYY2B008) （2024ZYY2B008）
