中国兽药杂志2025，Vol.59Issue(7)：35-39,5.DOI:10.11751/ISSN.1002-1280.2025.7.06
离子色谱法测定维生素B1中的硝酸根离子
Determination of Nitrate Ion in Vitamin B1 by Ion Chromatography
彭文绣 1赵富华 1张秀英 1韩宁宁 1王轩1
作者信息
摘要
Abstract
A method for content determination of nitrate ion in vitamin B1 was developed by ion chromatography with inhibitory electrical conductivity detector.Analysis was performed on a DIONEX IonPac AS19(250 mm×4 mm)anion exchange column and DIONEX IonPac AG19(50 mm×4 mm)guard column,using 14 mmol/L potassium hydroxide solution as the mobile phase.The flow rate was 1.0 mL/min and the column temperature was set at 35℃.Results showed that a good separation was accepted between nitrate ion and other conventional anions.The standard curve was linear in the range of 1～100 μg/mL(r2=1.0000).The average recovery was 100.8%(n=6,RSD=0.7%).In conclusion,this method is accurate,sensitive and specific for the determination of nitrate ion in vitamin B1.关键词
离子色谱/标准物质/维生素B1/硝酸根离子Key words
ion chromatography/standard substance/vitamin B1/nitrate ion分类
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彭文绣,赵富华,张秀英,韩宁宁,王轩..离子色谱法测定维生素B1中的硝酸根离子[J].中国兽药杂志,2025,59(7):35-39,5.
