中国兽药杂志2025，Vol.59Issue(7)：40-46,7.DOI:10.11751/ISSN.1002-1280.2025.7.07
麻芩止咳可溶性粉中盐酸麻黄碱含量测定法研究
Study on Quality Standard of Maqin Cough Soluble Powder
摘要
Abstract
In order to effectively control the quality of Maqin Cough Soluble Powder,high performance liquid chromatography(HPLC)detection method for determining the content of Maqin cough soluble powder was established based on ephedrine hydrochloride,the effective component of Ephedra,as an indicator,and the methodological inspection of the content was carried out.The results showed that the pretreatment method of the sample was to add hot water to dissolve it,add concentrated ammonia test solution and shake it with ether,then add hydrochloric acid ethanol solution,and recover the solvent to effectively extract the ephedrine hydrochloride contained in the sample;HPLC conditions,use octadecylsilane-bonded silica gel as filler,the mobile phase is acetonitrile-0.1%phosphoric acid solution(containing 0.1%triethylamine)(3∶97),the detection wavelength is 205 nm,and the injection volume is 10 μL.Under these conditions,blank interference determination was carried out on the negative preparation lacking Ephedra,and the results showed that there was no interference in the negative,ephedrine hydrochloride had a good linear relationship between 9.24 and 69.30 μg/mL,and the average recovery rate of sample addition was 99.59%.The precision,repeatability,solution stability,and durability all met the requirements.This shows that the established HPLC detection method for Maqin cough soluble powder is accurate and reliable,and can be used for quality control of Maqin Cough Soluble Powder,providing necessary conditions for the promotion and application of this product in clinical practice.关键词
麻芩止咳可溶性粉/高效液相色谱(HPLC)/盐酸麻黄碱/含量测定Key words
Maqin Cough Soluble Powder/high performance liquid chromatography(HPLC)/ephedrine hydrochloride/content determination分类
农业科技引用本文复制引用
张娜,陈静,郭莉,刘佳,张培训,张秋娜,李会平,孔红君..麻芩止咳可溶性粉中盐酸麻黄碱含量测定法研究[J].中国兽药杂志,2025,59(7):40-46,7.基金项目
"十四五"国家重点研发计划项目(2022YFD1801104) （2022YFD1801104）
