种子2025，Vol.44Issue(6)：10-16,31,8.DOI:10.16590/j.cnki.1001-4705.2025.06.010
黄淮麦区部分强筋小麦品质相关基因的KASP检测
KASP Detection of Quality Related Genes in Some Strong Gluten Wheat in the Huang-Huai Wheat Region
摘要
Abstract
In order to explore the composition of quality-related functional genes of some existing strong-gluten wheat(Triticum aestivum L.)varieties in the Huang-Huai Wheat Region,molecular detection was conducted on 13 functional genes related to quality traits,including high molecular weight glutenin gene,grain hardness gene,1BL/1RS translocation,yellow pigment content gene,polyphenol oxidase gene,peroxidase gene and adipoxygenase gene,of 26 representative strong-gluten wheat varieties in the Huang-Huai Wheat Region by using KASP labeling technology.The results showed that in the detection of high-molecular-weight glutenin Glu-A1,Glu-B1 and Glu-D1,the dis-tribution frequencies of superior allelic variations of the three genes were 88.5％,92.3％and 57.7％,respectively.Fourteen varieties including Zhengmai369 were all high-quality subunits at three loci,accounting for 53.8％.For the translocations of the grain hardness genes Pinb-D1 and 1BL/1RS,21 varieties including Zhongmai578 did not carry this translocation gene and were of the Pinb-D1b hard type,with a distribution frequency of 80.8％.For the four yellow pigment content loci Psy-B1,Psy-D1,Zds-A1 and Pds-B1,Xinmai26 and Zhoumai36 simultaneously carried four genes with low yellow pigment content(Psy-B1/Psy-D1/Zds-A1/Pds-B1),accounting for 7.7％of the total.For 11 varieties including polyphenol oxidase genes(Ppo-A1 and Ppo-D1)and peroxidase genes Pod-A1,Shiluan02-1 simultaneously polymerized 2 low polyphenol oxidase genes and 1 high peroxidase gene(Ppo-A1b/Ppo-D1a/Pod-A1b),accounting for 42.3％.The excellent allele Lox-B1a with high fat oxidase activity was not detected in all the tested materials.关键词
黄淮麦区/强筋小麦/品质基因/KASP检测Key words
Huang-Huai Wheat Region/strong gluten wheat/quality gene/KASP detection分类
农业科技引用本文复制引用
倪雪峰,谢红梅,孟自力,朱倩,闫向泉,王祁,吕侠雷,刘金栋,朱伟..黄淮麦区部分强筋小麦品质相关基因的KASP检测[J].种子,2025,44(6):10-16,31,8.基金项目
河南省农业(小麦)良种攻关项目"高产多抗小麦新品种选育与应用"(2022010103) （小麦）
河南省现代农业(小麦)产业技术体系项目"河南省现代农业产业技术体系专项资金项目"(Z2010-01-08) （小麦）
