种子2025，Vol.44Issue(6)：17-22,41,7.DOI:10.16590/j.cnki.1001-4705.2025.06.017
龙脑樟磷脂酸磷酸酶基因的克隆及表达分析
Cloning and Expression Analysis of the Phosphatidic Acid Phosphatase Genes from Cinnamomum camphora
摘要
Abstract
To explore the phosphatidic acid phosphatase gene CcPAP involved in borneol biosynthesis in Cinnamomum camphora,based on the genomic and transcriptome data of Cinnamomum campho-ra,of which three CcPAP genes were successfully cloned using PCR technology,with open reading frame lengths of 960 987 and 957 bp respectively,encoded proteins with lengths of 320 329 and 319 aa respectively,and was clustered together with the CsPAP of the Croton L.plants on the evolutionary tree.Bioinformatics analysis revealed that CcPAP1 was an unstable hydrophobic protein,while CcPAP3 and CcPAP8 were stable hydrophobic proteins.Three CcPAPs proteins were predicted to be located on chloroplasts through online software.The results of transcriptome and qRT-PCR analysis indicated that the expression levels of these three genes in different tissues of Cinnamomum camphora gradually decreased from the stem to the root and then to the leaf,and had a similar expression pat-tern to the borneol diphosphate synthase CcBPPS,suggesting that the three phosphatases were very likely to be involved in the biosynthesis of borneol in Cinnamomum cam phora.关键词
龙脑樟/磷脂酸磷酸酶/生物合成/龙脑Key words
Cinnamomum cam phora/phosphatidic acid phosphatase/biosynthesis/borneol分类
农业科技引用本文复制引用
杨泽锐,杨昕南,郑夏生..龙脑樟磷脂酸磷酸酶基因的克隆及表达分析[J].种子,2025,44(6):17-22,41,7.基金项目
国家自然科学基金青年基金项目(82304657) （82304657）
2024年度基础与应用基础研究专题"青年博士"启航"项目"(2024A04J4895) （2024A04J4895）
