种子2025，Vol.44Issue(6)：42-50,9.DOI:10.16590/j.cnki.1001-4705.2025.06.042
三环境基于水稻籽粒周长性状的QTL定位及候选基因分析
QTL Mapping and Candidate Gene Analysis of Rice Grain Circumference in Three Environments
摘要
Abstract
Based on the recombinant self-bred pedigres and high-density genetic linkage maps derived from the V20B/CPSLO17 genetic background,QTL scans and genetic effect analyses of rice(Oryza sativa L.)grain size traits were conducted in three environments(Guiyang in 2021,Guiyang in 2022,and Sanya in 2022)using IciMapping 4.0 software.The results showed that a total of 6 QTLs of grain circumference were detected in the three environments.Their LOD values ranged from 2.738 to 5.320,and the variation range of phenotypic contribution rate was 6.578％to 13.364％.2 QTLs(qGC3S-1 and qGC3-2)were genetically stable major QTLs for grain circumference.Their phenotypic contribution rates were both greater than 10％,and qGC3-1 was repeatedly tested four times,while qGC3-2 was repeatedly tested twice.The enhancer alleles of the four QTLs originated from the parental CPSLO17.The enhancer alleles of the 2 QTLs originated from the parental V20B.The grain circumference trait showed quantitative trait genetic characteristics regulated by multiple genes.The localization interval of QTL qGC3-2 had only one gene locus,while qGC4-1 contained two gene loci.The gene Os03g0406900,which was highly expressed in embryos and endosperm,was a candidate gene for QTL qGC3-2 in brown rice grains,and the gene Os04g0367400,which was highly expressed in glumes and glume,was a candidate gene for QTL qGC4-1 in grains.关键词
水稻/周长/基因位点/连锁图谱/定位区间Key words
Oryza sativa L./circumference/gene locus/linkage map/positioning interval分类
农业科技引用本文复制引用
彭强,吴健强,李佳丽,姜雪,张大双,朱速松..三环境基于水稻籽粒周长性状的QTL定位及候选基因分析[J].种子,2025,44(6):42-50,9.基金项目
贵州省基础研究(自然科学)项目"调控水稻籽粒大小的新基因的克隆研究"(黔科合基础-ZK[2023]一般178) （自然科学）
贵州省科技计划项目"贵州省优质特色水稻研发与转化及园区技术服务能力建设"(黔科合平台人才[2017]5719) （黔科合平台人才[2017]5719）
