种子2025，Vol.44Issue(6)：68-74,98,8.DOI:10.16590/j.cnki.1001-4705.2025.06.068
悬铃木LFY基因第二内含子多态性分析
Polymorphism Analysis of the Second Intron of LFY Gene in Platanus acerifolia
摘要
Abstract
The Platanus acerifolia is a hybrid of the Platanus occidentalis L.and the Platanus orientalis L.,showing obvious hybrid vigor and is widely used in landscaping.The LFY gene is a rare single-copy gene in dip-loid angiosperms.To explore the polymorphism of the second intron of the LFY gene in Platanus acerifolia,eight Platanus acerifolia samples were used as materials.Two pairs of primers,S257/S258 and S262/S263,were designed to amplify partial fragments of the second intron of the LFY gene respectively,and nucleotide sequence polymorphism analysis was conducted.The results indicated that based on SNP loci,primers S257/S258 could distinguish between Yuanke115,Yuanke23 and Sanqiu,and primers S262/S263 could distinguish between Yuanke23 and Sanqiu.In addition,a pair of SSR primers was developed for the 3′end GA repeat site of the second intron of the LFY gene in the Platanus occidentalis L.and was detected by capillary electrophoresis.The two LFY sequences of Yuanke10,Yuanke22 and Fast-growing Plane trees were respectively from the Pla-tanus occidentalis L.and Platanus orientalis L..The LFY of Yuanke115 and Yuanke23 both came from the Pla-tanus occidentalis L.,and the LFY of Yuanke12 all came from the Platanus orientalis L..关键词
悬铃木/LFY基因/内含子/SNP/SSRKey words
Platanus acerifolia/LFY gene/intron/SNP/SSR分类
农业科技引用本文复制引用
田娅林,张林霞,刘国锋,江杰,张思思..悬铃木LFY基因第二内含子多态性分析[J].种子,2025,44(6):68-74,98,8.基金项目
国家自然科学基金项目"AGL17-like基因对矮牵牛开花的调控功能与机制研究"(32102425) （32102425）
武汉市园林和林业局科技计划项目"悬铃木TFL1同源基因抑制矮牵牛开花的分子机理"(WHGF2022A09) （WHGF2022A09）
