浙江大学学报（医学版）2025，Vol.54Issue(3)：333-341,9.DOI:10.3724/zdxbyxb-2024-0355
茯苓酸可促进前脂肪细胞棕色化及脂代谢
Pachymic acid promotes brown/beige adipocyte differentiation and lipid metabolism in preadipocytes
摘要
Abstract
Objective:To investigate the effect of pachymic acid on brown/beige adipocyte differentiation and lipid metabolism in preadipocytes.Methods:3T3-L1 MBX cells were induced to differentiate into beige adipocytes using a brown cocktail method.The impact of pachymic acid on the viability of 3T3-L1 MBX cells was evaluated using the CCK-8 assay.The formation of lipid droplets following treatment with pachymic acid was observed by oil red O staining.The mRNA expression levels of key browning genes,including uncoupling protein(Ucp)1,the peroxisome proliferator activated receptor-γcoactivator(Pgc)-1α,and the PR domain-containing protein 16(Prdm16),as well as the mRNA expression of sterol regulatory element-binding protein(Srebp)1c,acetyl-coA carboxylase(Acc),fatty acid synthase(Fas),and hormone-sensitive triglyceride lipase(Hsl),adipose triglyceride lipase(Atgl),and carnitine palmitoyltransferase(Cpt)1 were detected by quantitative reverse transcription polymerase chain reaction.The protein expression of Ucp1,Pgc-1a,and Prdm16 was detected by Western blotting.Results:The 3T3-L1 MBX cells were induced in vitro to form beige adipocytes with high expression of key browning genes(Ucp1,Pgc-1α,and Prdm16),and beige adipose-marker genes(Cd137,Tbx1,and Tmem26).Concentrations range of 0-80 μmol/L pachymic acid were non-cytotoxic to 3T3-L1 MBX cells.Pachymic acid treatment significantly inhibited the differentiation of 3T3-L1 MBX cells,resulting in a notable decrease in lipid accumulation.There was a marked increase in the expression of key browning genes and their proteins products,such as Ucp1,Pgc-1α,and Prdm16,while the expressions of fat synthesis-related genes Srebp1c,Acc and Fas were significantly decreased(all P＜0.05).The expressions of lipolysis-related genes(Hsl,Atgl,and Cpt1)were significantly increased(all P＜0.05).Treatment with 20 μmol/L pachymic acid showed the most pronounced effect.Conclusion:Pachymic acid can inhibit fat synthesis and promote lipid decomposition by regulating the brown formation and lipid differentiation of preadipocytes.关键词
茯苓酸/3T3-L1 MBX细胞/脂肪细胞棕色化/成脂分化/脂代谢Key words
Pachymic acid/3T3-L1 MBX cells/Adipocyte browning/Lipogenic differentiation/Lipid metabolism分类
医药卫生引用本文复制引用
陈坤铃,窦晓兵,林益游,白丹瑶,罗杨舟,周丽萍..茯苓酸可促进前脂肪细胞棕色化及脂代谢[J].浙江大学学报（医学版）,2025,54(3):333-341,9.基金项目
国家自然科学基金(82374102) （82374102）
浙江中医药大学科研项目(2022JKZKTS21) The study was supported by the National Natural Science Foundation of China(82374102)and Scientific Research Program of Zhejiang University of Traditional Chinese Medicine(2022JKZKTS21) （2022JKZKTS21）
