浙江农业学报2025，Vol.37Issue(6)：1212-1220,9.DOI:10.3969/j.issn.1004-1524.20240541
bta-miR-146b对热应激奶牛乳腺上皮细胞乳蛋白合成信号通路的影响
Effects of bta-miR-146b on the signaling pathway of milk protein synthesis in heat-stressed bovine mammary epithelial cells
摘要
Abstract
To investigate the impact of bta-miR-146b on milk protein synthesis in dairy cows under heat stress,bo-vine mammary epithelial cells(BMECs)were transfected with bta-miR-146b mimics,bta-miR-146b inhibitor,bta-miR-146b mimics negative control(NC)and bta-miR-146b inhibitor NC.Following transfection,the cells were sub-jected to heat stress(40 ℃).The relative expression levels of milk protein-related genes(CSN2,CSN3)and genes associated with milk protein synthesis signaling pathways(JAK2,STAT5,mTOR,MAPK)in each group were detec-ted using quantitative real-time PCR(qRT-PCR).This allowed for analysis of the effects of bta-miR-146b overex-pression and inhibition on milk proteins and their synthesis signaling pathways under both heat stress and non-heat stress conditions.The results showed that:Transfection with the bta-miR-146b mimics significantly increased bta-miR-146b expression in BMECs,while transfection with the inhibitor significantly decreased its expression;Heat stress significantly suppressed the expression of CSN2,CSN3,JAK2,STAT5,mTOR and MAPK genes in the blank control group,the bta-miR-146b mimic group,and the bta-miR-146b inhibitor group;Under non-heat stress condi-tions,overexpression of bta-miR-146b significantly decreased the expression levels of CSN2,CSN3,JAK2,STAT5,mTOR and MAPK genes,while inhibition of bta-miR-146b significantly increased the expression of CSN3,JAK2,STAT5,mTOR and MAPK genes.Under heat stress conditions,overexpression of bta-miR-146b significantly de-creased the expression levels of CSN2,CSN3,JAK2,STAT5,mTOR and MAPK genes,while inhibition of bta-miR-146b significantly increased the expression of these genes.In summary,both heat stress treatment and overexpression of bta-miR-146b significantly inhibit the expression of genes related to milk proteins and their synthesis signaling pathways.Conversely,inhibition of bta-miR-146b expression promotes the expression of these genes.关键词
奶牛/热应激/乳蛋白/信号通路/表达量Key words
dairy cow/heat stress/milk protein/signaling pathway/expression level分类
农业科技引用本文复制引用
张悦宇,黄美琦,张琳,齐颖,李秋玲..bta-miR-146b对热应激奶牛乳腺上皮细胞乳蛋白合成信号通路的影响[J].浙江农业学报,2025,37(6):1212-1220,9.基金项目
河北省高等学校科学技术重点研究项目(ZD2021091) （ZD2021091）
河北省属高校基本科研业务费项目—优秀创新团队(JYT202301) （JYT202301）
河北省属高校基本科研业务费项目—优秀青年项目(JYQ202301) （JYQ202301）
大学生创新创业训练计划项目(X202310100024) （X202310100024）
