浙江农业学报2025，Vol.37Issue(6)：1221-1232,12.DOI:10.3969/j.issn.1004-1524.20240499
嗜水气单胞菌胁迫下棘胸蛙脾脏的病理损伤
Pathological lesions in spleen of Chinese spiny frog(Quasipaa spinosa)after Aeromonas hy-drophila infection
摘要
Abstract
In order to explore the pathological lesions in spleen of Chinese spiny frog(Quasipaa spinosa)after haem-orrhagic septicemia infection,Aeromonas hydrophila isolated from diseased Chinese spiny frog,which was identified as the pathogenic bacterium,was used to replicate haemorrhagic septicemia in health individual with body weight of(130±20)g.The challenge concentration was 1 × 107 CFU·mL-1 and the injected dose was 0.2 mL per frog.The spleen was selected as representative of the internal organs for clinical symptoms and dynamic pathological observa-tion was carried out at 2 h,4 h,8 h,12 h,16 h,24 h,32 h,40 h,48 h,60 h,72 h,96 h(4 d),120 h(5 d),144 h(6d),168 h(7 d)after infection,respectively.It was shown that the main clinical signs included lethargy and depression with increasing of the spleen index which was significantly(P＜0.01)different between the control group and the infection group from 40 h to 5 d.The observation of dynamic pathological showed necrosis and reduc-tion of lymphocytes and reticular cells in white pulp district with an increase of single melano-macrophage,and var-ying degrees of degeneration and necrosis in tunica.Especially,the typical pathological damage included severe nec-rosis in white pulp and disappearance of melano-macrophage centres(MMCs)with reticular fibre decomposition from 48 h to 72 h.The research indicated that under the stress of lethal septicemia,the splenic structure of the frog was disintegrated,the cell components were severely necrotic,and the structural basis of the immune organ was de-stroyed.关键词
棘胸蛙/嗜水气单胞菌/致死性败血症/病理损伤Key words
Quasipaa spinosa/Aeromonas hydrophila/haemorrhagic septicaemia/pathological lesions分类
农业科技引用本文复制引用
任思宇,吴春艳,刘震坤,杨延辉..嗜水气单胞菌胁迫下棘胸蛙脾脏的病理损伤[J].浙江农业学报,2025,37(6):1221-1232,12.基金项目
重庆市教委科研项目(KJQN202203514) （KJQN202203514）
万州区科技局博士直通车项目(wzstc20220120) （wzstc20220120）
重庆三峡职业学院创新团队(sxzy-2023td-006) （sxzy-2023td-006）
