浙江农业学报2025，Vol.37Issue(6)：1285-1292,8.DOI:10.3969/j.issn.1004-1524.20240530
GTP环化水解酶——一种防治褐飞虱(Nilaparvata lugens)的RNAi靶标基因
GTP cyclohydrolase:a RNAi target gene against the brown planthopper(Nilaparvata lu-gens)
摘要
Abstract
Guanosine triphosphate cyclohydrolase Ⅰ(GCHI)is a crucial regulatory factor for the growth and develop-ment of holometabolous insects,involved in melanin synthesis and the molting process.To evaluate the feasibility of the GCHI gene as a target for RNA interference(RNAi)in controlling the hemimetabolous insect Nilaparvata lugens(brown planthopper,BPH),this study cloned the NlGCHI gene from BPH,analyzed its spatiotemporal expression patterns,and validated gene function through microinjection experiments.The results indicated that NlGCHI has two transcripts,designated as NlGCHIa and NlGCHIb.Notably,NlGCHIb exhibited periodic expression dynamics in late-instar nymphs and was highly expressed in the cuticle.Treatment of BPH with dsNlGCHI injection or feeding with the GCHI inhibitor 3-deoxy-D-arabino-heptulosonate-7-phosphate(DAHP)resulted in high mortality rates,primarily due to molting defects characterized by failure to shed the old cuticle properly.Additionally,dsNlGCHI-treated indi-viduals displayed abnormal melanization.The above results indicate that the function of GCHI remains relatively con-served in both holometabolous insects and hemimetabolous insect,influencing cuticle formation and molting processes in insects.Its encoding gene can be utilized to develop RNAi-based pesticides for controlling N.lugens.关键词
褐飞虱/RNA干扰/磷酸鸟苷环化水解酶Ⅰ/表皮Key words
Nilaparvata lugens/RNA interference/guanosine triphosphate cyclohydrolase Ⅰ/cuticle分类
农业科技引用本文复制引用
董代幸,羊桂英,王爱英,罗举,刘淑华..GTP环化水解酶——一种防治褐飞虱(Nilaparvata lugens)的RNAi靶标基因[J].浙江农业学报,2025,37(6):1285-1292,8.基金项目
浙江省农业产业技术团队项目 （）
