浙江医学2025，Vol.47Issue(13)：1357-1364,8.DOI:10.12056/j.issn.1006-2785.2025.47.13.2025-537
丹酚酸A联合紫杉醇通过脂代谢抑制前列腺癌恶性生物学行为的作用研究
Role of Salvianolic acid A combined with paclitaxel in inhibiting the malignant biological behavior of prostate cancer through lipid metabolism
摘要
Abstract
Objective To investigate the effect of salvianolic acid A(SalA)combined with paclitaxel on inhibiting the malignant biological behaviors of prostate cancer(PCa)through lipid metabolism.Methods PCa cell lines PC3 and 22RV1 were used,and a control group(complete medium),a SalA group(PC3:80 μmol/L;22RV1:20 μmol/L),a paclitaxel group(PC3:40 nmol/L;22RV1:10 nmol/L),and a combination group(PC3:80 μmol/L SalA+40 nmol/L paclitaxel;22RV1:20 μmol/L SalA+10 nmol/L paclitaxel)were set.After 24 h of drug treatment,cell counting kit-8 method,cell clone formation experiment,scratch healing experiment,and Transwell invasion experiment were used to detect PCa cell activity,proliferation,migration,and invasion abilities respectively;annexin V-fluorescein isothiocyanate/propidium iodide double staining was used to detect cell apoptosis rate;Nile red staining was used to detect lipid droplet fluorescence intensity;free fatty acid(FFA)detection kit was used to quantitatively detect FFA content;protein imprinting method(Western blot)was used to detect expressions of apoptosis-related proteins including matrix metalloproteinase-9(MMP-9),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),and lipid metabolism-related proteins including long-chain acyl-coenzyme A synthase 4(ACSL4).Results SalA combined with paclitaxel synergistically inhibited PCa cell activity,proliferation,migration,and invasion abilities,significantly induced cell apoptosis(all P＜0.05),while down-regulated MMP-9 and Bcl-2 expressions and up-regulating Bax expression(all P＜0.05).SalA enhanced the reprogramming effect of paclitaxel on lipid metabolism,manifested as reduced lipid droplet fluorescence intensity,FFA content,and ACSL4 expression(all P＜0.05);SalA combined with paclitaxel synergistically reduced lipid droplet fluorescence intensity and FFA content in PCa cells(both P＜0.05),while down-regulated ACSL4 expression(P＜0.05).Conclusion SalA enhances the chemotherapy sensitivity of PCa cells to paclitaxel through lipid metabolism reprogramming.关键词
前列腺癌/紫杉醇/丹酚酸A/细胞凋亡/脂代谢Key words
Prostate neoplasms/Paclitaxel/Salvianolic acid A/Cell apoptosis/Lipid metabolism引用本文复制引用
沈立亮,金妍杉,陆晶晶,张逸..丹酚酸A联合紫杉醇通过脂代谢抑制前列腺癌恶性生物学行为的作用研究[J].浙江医学,2025,47(13):1357-1364,8.基金项目
宁波市眼科临床医学研究中心项目(2022L003) （2022L003）
