中兽医医药杂志2025，Vol.44Issue(4)：33-41,9.DOI:10.13823/j.cnki.jtcvm.2025.042
中药饲料添加剂预防羔羊腹泻临床试验研究
Chinese herbal feed additives for the prevention of diarrhea in lambs:a clinical trial
摘要
Abstract
This trial aimed to investigate the preventive effect of a Chinese herbal feed additive on diarrhea in suckling lambs.Fifty-six healthy 7-day-old Hu lambs(approximately 5 kg body weight)were selected and randomly allocated into four groups:a control group,and trial groupsⅠ,Ⅱ,and Ⅲ.The groups were supplemented with 0％,1％,2％,and 4％of a compound Chinese herbal additive(composed of Zingiber officinale,Pueraria montana var.lobata and Aucklandia costus,etc)in their starter feed,respectively,for a 45-day feeding period.Diarrhea incidence was observed and recorded daily to calculate the diarrhea rate.Body weight and feed intake were measured every 15 days to calculate average daily gain(ADG)and feed conversion ratio(FCR).Blood samples were collected concurrently every 15 days;plasma was separated and used to measure biochemical indices(TP,ALB,ALT,CRE)on a microplate reader using enzymatic methods(bromocresol green method,BCA method,colorimetric method and sarcosine oxidase method).Antioxidant parameters(GSH-Px,T-AOC,SOD,MDA)were determined on a microplate reader/spectrophotometer using specific assay principles(visible chromogenic method,FRAP method,WST-8 method and microplate/TBA method).Immune markers(IgG,IFN-γ,IL-1β,IL-6,IL-10,TNF-α)were quantified using a double-antibody sandwich enzyme-linked immunosorbent assay(ELISA)with specific commercial kits and a microplate reader.The results showed that the diarrhea rates of lambs in group Ⅱ and Ⅲ were extremely significantly lower than that in the control group(P<0.01);there were no significant differences in body mass,feed intake,average daily gain,or feed conversion ratio among the groups(P>0.05),but groupⅡshowed relatively better performance in these indicators.On day 45,plasma ALB levels in all three experimental groups were extremely significantly higher than that in the control group(P<0.01),and ALT levels were significantly lower than that in the control group(P<0.05).On day 15,significant changes in plasma antioxidant indices began to appear in the experimental groups compared to the control group.On day 45,GSH-Px,SOD,T-AOC and MDA in all three experimental groups differed significantly from the control group(P<0.05),with groupⅡ being significantly better than groups Ⅰ andⅢ.On day 15,30 and 45,plasma IgG and IFN-γ levels in all three experimental groups were extremely significantly higher than that of the control group(P<0.01),the anti-inflammatory factor IL-10 level in group Ⅱ was extremely significantly higher than that of the other groups(P<0.01),while the levels of pro-inflammatory factors(IL-1β,IL-6,TNF-α)were extremely significantly lower than that of the other groups(P<0.01).Therefore,adding this Chinese herbal feed additive to lamb starter feed can enhance antioxidant function and regulate immune function,thereby reducing the diarrhea rate without affecting diet palatability or feed intake.A 2％inclusion level is recommended as the optimal dietary dose.These results provide a scientific basis for the development of Chinese herbal additives to prevent lamb diarrhea.关键词
中药饲料添加剂/羔羊/腹泻/生长性能/抗氧化/免疫调节Key words
Chinese herbal additives/lambs/diarrhea/growth performance/antioxidant function/immunoregulation分类
农业科技引用本文复制引用
王际中,魏赛,张洺溪,韩鑫,李陇平,毕台飞,屈雷,董书伟..中药饲料添加剂预防羔羊腹泻临床试验研究[J].中兽医医药杂志,2025,44(4):33-41,9.基金项目
陕西省科技厅一般项目-农业领域(S2023-YF-YBNY-0080) （S2023-YF-YBNY-0080）
榆林学院高层次人才引进项目(18GK10) （18GK10）
榆林市科技项目(CXY-2020-011-05) （CXY-2020-011-05）
