作物学报2025，Vol.51Issue(8)：1981-1990,10.DOI:10.3724/SP.J.1006.2025.53010
戈壁异常球菌csp2基因提高玉米的抗旱性
csp2 gene of Deinococcus gobiensis improves drought tolerance in maize
摘要
Abstract
The csp2 gene,derived from Deinococcus gobiensis,is known for its strong resistance to environmental stressors such as gamma radiation,UV light,and drought.In this study,the csp2 gene was codon-optimized for plant expression,synthesized,and inserted into the plant expression vector p3301-csp2,which was subsequently introduced into maize.The drought tolerance of the csp2 transgenic lines was evaluated at both the seedling and adult stages.Under drought conditions,the csp2 overexpression lines exhibited significantly enhanced drought tolerance at the seedling stage compared to wild-type plants,as evidenced by higher relative water content and markedly reduced electrolyte leakage,malondialdehyde(MDA)levels,and hydrogen peroxide(H2O2)accumulation.At the adult stage,drought stress assessments demonstrated that csp2 expression increased ear length and single-ear weight,resulting in significantly higher yields in transgenic lines compared to the wild type.Transcriptome analysis revealed that csp2 enhances drought tolerance by modulating the expression of genes involved in the jasmonic acid signaling pathway,drought-responsive kinases,and the WRKY and ERF transcription factor families.These findings suggest that heterolo-gous expression of Deinococcus gobiensis csp2 significantly improves drought tolerance in maize,making csp2 a promising can-didate gene for drought-resilient crop breeding.关键词
csp2/戈壁异常球菌/转基因玉米/耐旱/RNA-seqKey words
csp2/Deinococcus gobiensis/transgenic maize/drought tolerance/RNA-seq引用本文复制引用
许忆葳,张莹莹,李瑞,燕永亮,刘允军,孔照胜,郑军,王逸茹..戈壁异常球菌csp2基因提高玉米的抗旱性[J].作物学报,2025,51(8):1981-1990,10.基金项目
本研究由国家重点研发项目(2023YFD1200500)和新疆维吾尔自治区自然科学基金项目(2022D01D87)资助.This study was supported by the National Key R&D Program of China(2023YFD1200500)and the Natural Science Foundation of Xinjiang Uygur Autonomous Region(2022D01D87). （2023YFD1200500）
