作物学报2025，Vol.51Issue(8)：1991-2008,18.DOI:10.3724/SP.J.1006.2025.55013
大豆不同生育时期耐盐性综合评价及耐盐种质筛选
Comprehensive evaluation of salt tolerance at different growth stages of soy-bean and screening of salt-tolerant germplasm
摘要
Abstract
Soil salinization is a major abiotic stress that severely hampers soybean growth and productivity.The evaluation and selection of salt-tolerant soybean germplasm are essential for identifying salt-alkali tolerance genes,breeding salt-tolerant culti-vars,and improving the efficient use of saline-alkali soils.In this study,50 soybean germplasm accessions were evaluated for salt tolerance at three developmental stages:germination(0.6%NaCl),seedling(1.5%NaCl),and the full growth period(0.9%NaCl).Salt tolerance was assessed using germination rate at the germination stage;at the seedling stage,ten physiological and morpho-logical indicators were measured,including plant height,leaf area,SPAD value,fresh and dry weights of shoots and roots,and malondialdehyde content.During the full growth stage,eight agronomic traits were evaluated,such as plant height,pod height,number of effective branches,pods per plant,seeds per plant,and seed weight per plant.A comprehensive evaluation was con-ducted using correlation analysis,principal component analysis(PCA),membership function analysis,and cluster analysis.Step-wise regression was employed to construct predictive models for salt tolerance at the seedling and full-growth stages,identifying key evaluation indicators for each stage.Based on the salt injury index,8 highly salt-tolerant,10 salt-tolerant,and 6 highly salt-sensitive germplasms were identified at the germination stage.PCA combined with the membership function approach identi-fied 12 salt-tolerant accessions at the seedling stage.Cluster analysis grouped the 50 accessions into five categories during the full growth stage:3 highly salt-tolerant,3 salt-tolerant,20 moderately salt-tolerant,19 salt-sensitive,and 5 highly salt-sensitive.The regression model for salt tolerance at the seedling stage was defined as:D=-0.223+0.085X1+0.203X2+0.075X3+0.149X6+0.132X7+0.070X9+0.084X10(R2=0.969,P<0.01),identifying relative plant height,leaf area,SPAD value,root fresh and dry weights,SOD activity,and proline content as key indicators.For the full growth stage,the model was:D=-0.153+0.143X1+0.443X6+0.171X7(R2=0.962,P<0.01),highlighting relative plant height,grain number per plant,and grain weight per plant as critical metrics.This study establishes a comprehensive and systematic framework for evaluating salt tolerance in soybean across different developmental stages and provides a solid technical foundation and valuable germplasm resources for future research on salt tolerance mechanisms and the development of salt-tolerant soybean varieties.关键词
大豆/发芽期/苗期/全生育期/耐盐性/隶属函数法/综合评价Key words
soybean/germination stage/seedling stage/entire growth stage/salt tolerance/membership function method/com-prehensive evaluation引用本文复制引用
孟然,李赵嘉,冯薇,陈悦,刘路平,杨春燕,鲁雪林,王秀萍..大豆不同生育时期耐盐性综合评价及耐盐种质筛选[J].作物学报,2025,51(8):1991-2008,18.基金项目
本研究由唐山市科技计划项目(23150207A)和河北省农林科学院现代农业科技创新工程课题项目(2022KJCXZX-BHS-4)资助.This study was supported by the Tangshan Science and Technology Plan Project(23150207A)and the HAAFS Science and Technology Innovation Special Project(2022KJCXZX-BHS-4). （23150207A）
