作物学报2025，Vol.51Issue(8)：2048-2059,12.DOI:10.3724/SP.J.1006.2025.54014
蔗茅耐寒相关基因EfWRKY51克隆分析及功能验证
Cloning analysis and functional validation of EfWRKY51 gene related to cold tolerance in Erianthus fulvus
摘要
Abstract
WRKY family transcription factors play critical roles in plant response to cold stress.Erianthus fulvus,a wild relative of sugarcane,exhibits remarkable cold tolerance;however,the function of WRKY genes in cold stress its response remains largely unknow.In this study,the EfWRKY51 gene(GenBank accession no:UVJ69259.1)was cloned from E.fulvus.The full-length cod-ing sequence(CDS)was 915 bp and encoding a 304-amino-acid protein.EfWRKY51 expression was upregulated under cold stress,and the encoded protein localized to the nucleus but exhibited no ptranscriptional activation activity.Transgenic tobacco lines overexpressing EfWRKY51 were generated via Agrobacterium-mediated leaf disc transformation.Following cold treatment,transgenic lines showed less leaf wilting compared to the wild type(WT),and displayed significantly higher seed germination rates and root lengths.Moreover,under cold stress,transgenic lines exhibited significantly higher activities of peroxidase(POD)and superoxide dismutase(SOD),as well as increased levels of proline(Pro)and soluble sugars(SS),while malondialdehyde(MDA)content was significantly reduced compared to WT.These results indicate that EfWRKY51 overexpression enhances cold tolerance in transgenic tobacco.This study provides new insights into the regulatory role of EfWRKY51 under cold stress and of-fers a valuable genetic resource for improving cold tolerance in sugarcane.关键词
甘蔗/蔗茅/EfWRKY51基因/异源表达/耐寒性Key words
sugarcane/Erianthus fulvus/EfWRKY51 gene/heterologous expression/cold tolerance引用本文复制引用
万慧兰,吴华英,曾丹,钱禛锋,赵昌祖,廖然超,何丽莲,李富生..蔗茅耐寒相关基因EfWRKY51克隆分析及功能验证[J].作物学报,2025,51(8):2048-2059,12.基金项目
本研究由云南省重大科技专项(202202AE090021)和国家自然科学基金项目(31960451)资助.This study was supported by the Major Science and Technology Project of Yunnan Province(202202AE090021)and the National Natural Science Foundation of China(31960451). （202202AE090021）
