作物学报2025，Vol.51Issue(8)：2087-2099,13.DOI:10.3724/SP.J.1006.2025.42018
水稻黄单胞菌噬菌体vB_XaS_HDB2的全基因组分析和生物学特性研究
Whole genome analysis and biological characterization of phage vB_XaS_HDB2 infected with Xanthomonas oryzae pv.oryzae
摘要
Abstract
Bacterial leaf blight(BLB)of rice,caused by Xanthomonas oryzae pv.oryzae(Xoo),is a widespread and destructive disease that significantly reduces rice yield and threatens global food security.Currently,few chemical agents are available for BLB control,and most are either ineffective or environmentally hazardous.Therefore,there is an urgent need to develop novel,safe,and effective antibacterial alternatives.Bacteriophages,due to their high specificity against bacterial hosts,have emerged as promising biocontrol agents.In this study,we isolated a lytic phage targeting Xanthomonas,designated vB_XaS_HDB2(HDB2),from rice and shrimp field water.Transmission electron microscopy revealed that HDB2 is a long-tailed phage with a head diameter of(48±3)nm and a tail length of(166±8)nm.Whole-genome sequencing showed that HDB2 has a genome size of 43,697 bp,a GC content of 54.31%,and encodes 52 open reading frames(ORFs),of which 30 were functionally annotated and grouped into four modules:DNA metabolism,lysis,packaging,and structural assembly.Notably,HDB2 carries one tRNA gene but no virulence or antibiotic resistance genes.Comparative analyses,including average nucleotide identity(ANI),protein net-work mapping,and phylogenetic tree construction,revealed that HDB2 belongs to the genus Septimatrevirus and is most closely related to phage vB_Xar_IVIA-DoCa8(97.74%similarity).HDB2 was capable of lysing 52.9%(9/17)of tested Xanthomonas strains.One-step growth curve analysis indicated a latent period of 3 hours,a lysis period of 5 h,and a burst size of 44 PFU per cell.HDB2 also demonstrated strong stability across a wide temperature range(4-60℃)and pH range(4-11).In vitro experi-ments showed that HDB2 effectively inhibited the growth of Xoo strain 2086 at multiplicities of infection(MOIs)greater than 0.1.Collectively,these results highlight the potential of HDB2 as a biocontrol agent and provide a theoretical foundation for the ap-plication of phage-based strategies in managing bacterial diseases in crops.关键词
水稻黄单胞菌/水稻白叶枯病/噬菌体/全基因组分析/生物学特性Key words
Xanthomonas oryzae pv.oryzae/bacterial leaf blight of rice/phage/whole genome analysis/biological characteristics引用本文复制引用
陈惠莹,何嘉欣,朱斌,黄士轩,周星佑,伍君权,杨美艳..水稻黄单胞菌噬菌体vB_XaS_HDB2的全基因组分析和生物学特性研究[J].作物学报,2025,51(8):2087-2099,13.基金项目
本研究由国家自然科学基金项目(32202194),广东省重点领域研发计划项目(2022B0202110001)和广州市重点研发计划农业和社会发展科技专题项目(SL2022B03J01243)资助.This study was supported by the National Natural Science Foundation of China(32202194),the Guangdong Key Areas Research and Deve-lopment Program Project(2022B0202110001),and the Guangzhou Key Research and Development Program Agricultural and Social Deve-lopment Science and Technology Project(SL2022B03J01243). （32202194）
