作物学报2025，Vol.51Issue(8)：2220-2227,8.DOI:10.3724/SP.J.1006.2025.44203
LsSAT2与LsAAE3互作调控山黧豆ODAP水平
Regulation of ODAP levels in Lathyrus sativus L.via interaction between LsSAT2 and LsAAE3
摘要
Abstract
Serine acetyltransferase 2(LsSAT2)in Lathyrus sativus functions as the rate-limiting enzyme in the biosynthesis of the neu-roactive compound β-ODAP(β-N-oxalyl-L-α,β-diaminopropionic acid).To explore the regulatory mechanisms underlying LsSAT2 activ-ity,we overexpressed LsSAT2 in L.sativus hairy roots.Proteins interacting with LsSAT2 were subsequently identified via immunopre-cipitation followed by mass spectrometry(IP-MS),and their interactions were confirmed using protein-protein docking,yeast two-hybrid(Y2H)analysis,and pull-down assays.The results revealed that LsSAT2 interacts with an acyl-CoA synthetase,LsAAE3,through its C-terminal region.Overexpression of LsSAT2 or LsAAE3 in hairy roots led to a 52.4%reduction or a 55.9%increase in β-ODAP content,respectively,indicating a functional interplay between these two proteins.These findings provide important insights into the genetic regu-lation of β-ODAP biosynthesis and establish a foundation for future metabolic engineering in L.sativus.关键词
山黧豆/三七素/丝氨酸乙酰基转移酶/草酰辅酶A合成酶/蛋白互作Key words
Lathyrus sativus/dencichine/serine acetyltransferase/oxalyl-CoA synthase/protein-protein interaction引用本文复制引用
刘晓宁,张颖,蔡曼蕾,马昊,苗智博,曹宁,连荣芳,徐全乐..LsSAT2与LsAAE3互作调控山黧豆ODAP水平[J].作物学报,2025,51(8):2220-2227,8.基金项目
本研究由河南省教育厅高等学校重点科研项目(25B180013),陕西省自然科学基金项目(2023-JC-YB-152),财政部和农业农村部国家现代农业产业技术体系建设专项(食用豆,CARS-08-Z21),河南省科技攻关项目(252102110271)和国家级大学生创新创业训练计划项目(202401360B1)资助.This study was supported by the Key Scientific Research Projects of Henan Provincial Department of Education for Institutions of Higher Learning(25B180013),the Natural Science Foundation of Shaanxi Province(2023-JC-YB-152),the China Agriculture Research System of MOF and MARA(Food Legumes,CARS-08-Z21),the Tackling-Plan Project of Henan Department of Science and Technology(252102110271),and the National Undergraduate Training Programs fo Innovation and Entrepreneurship(202401360B1). （25B180013）
