作物学报2025，Vol.51Issue(8)：2228-2239,12.DOI:10.3724/SP.J.1006.2025.44159
大豆e1-as基因突变体的创制及生理分析
Creation and physiological analysis of an e1-as gene mutant in soybean
摘要
Abstract
Glycine max is a photoperiod-sensitive plant,and E1 is a key gene regulating flowering time in soybean.As a core component of the photoperiodic flowering pathway,E1 plays a critical role in controlling the timing of floral transition.In this study,we constructed a CRISPR/Cas9-based genome editing vector targeting the E1 gene and introduced it into the soybean cul-tivar Williams 82.Gene editing was successfully detected in the E1 gene of T1 generation plants,and T2 generation plants were subsequently developed.Sequencing analysis revealed a single base insertion at target site 1 in the E1 gene,resulting in a prema-ture stop codon and truncated protein.Phenotypic analysis showed that the flowering time of the T3 generation was approximately(10±2)days earlier than that of the wild type.In addition,chlorophyll content in the newly emerged trifoliate leaves of the mutant was(6±2)µg g-1 lower than in the wild type,while in fully expanded leaves,it was(10±4)µg g-1 lower.The pollen germination rate in the mutant was 8％±1％lower compared to the wild type.Furthermore,mutant pollen tube lengths measured at 0.25,0.50,1.00,and 2.00 hours were(33.96±5.00),(74.14±5.00),(142.86±5.00),(183.50±5.00)µm,respectively,whereas the corresponding values in the wild type were(46.08±5.00),(118.89±5.00),(228.35±5.00),and(307.72±5.00)µm.Transcriptome analysis of the e1-as-79 mutant and the wild type identified 3615 differentially expressed genes,including key flowering regulators FT2a and FT5a,which likely contribute to the early flowering phenotype observed in the mutant.关键词
大豆/E1基因/基因编辑/突变体/转录组Key words
soybean/E1 gene/gene editing/mutant/transcriptome引用本文复制引用
贺红利,张雨涵,杨静,程云清,赵杨,李星诺,司洪亮,张兴政,杨向东..大豆e1-as基因突变体的创制及生理分析[J].作物学报,2025,51(8):2228-2239,12.基金项目
本研究由国家自然科学基金项目(32101649)和吉林省科技创新创业卓越人才(团队)项目(20240601063RC)资助.This study was supported by the National Natural Science Foundation of China(32101649)and the Outstanding Talents Team Project of Department of Science and Technology of Jilin Province(20240601063RC). （32101649）
