植物保护学报2025，Vol.52Issue(3)：545-556,12.DOI:10.13802/j.cnki.zwbhxb.2025.2025028
农业害虫环境DNA监测技术的标准化研究现状与展望
Current status and prospects of standardizing eDNA-based monitoring for agricultural pests
摘要
Abstract
Environmental DNA(eDNA)technology,known for its high sensitivity and non-invasive sampling,shows great promise for monitoring agricultural pests.While notable progress has been made in standardizing eDNA applications within natural ecosystems(including aquatic and terrestrial ecosys-tems),standardization within agricultural ecosystems remains comparatively limited.This review focuses on pest monitoring in agricultural settings,comparing practices across key stages such as field sampling,sample processing,and molecular analysis between aquatic organisms and agricultural pests.Despite its potential,eDNA-based pest monitoring faces challenges in areas such as protocol develop-ment,laboratory workflow optimization,and quality control.In response,this article outlines future directions including:(1)establishing comprehensive,standardized operating procedures;(2)promoting synergistic use of eDNA with conventional pest surveillance methods,and(3)enhancing the integration of monitoring results into pest control decision-making.These recommendations aim to promote the standardized adoption of eDNA technology and support sustainable pest control strategies in agricul-tural systems.关键词
农业害虫/环境DNA/标准化/监测Key words
agricultural pest/environmental DNA(eDNA)/standardization/monitoring引用本文复制引用
刘莹,刘雅歌,甘力月,褚栋..农业害虫环境DNA监测技术的标准化研究现状与展望[J].植物保护学报,2025,52(3):545-556,12.基金项目
国家重点研发计划(2021YFD1400200),青岛市科技惠民示范专项(24-1-8-xdny-10-nsh),山东省农业重大技术协同推广计划(SDNYXTTG-2024-38) （2021YFD1400200）
