植物保护学报2025，Vol.52Issue(3)：591-602,12.DOI:10.13802/j.cnki.zwbhxb.2025.2025025
梨小食心虫取食后梨果的代谢响应及茉莉酸类物质对其行为的调控作用
Metabolic responses of pear fruit to oriental fruit moth Grapholita molesta herbivory and behavioral regulation by jasmonates
摘要
Abstract
To elucidate metabolic responses of pear fruit induced by oriental fruit moth Grapholita molesta herbivory and identify behaviorally active compounds involved in this interaction,metabolomic profiling was conducted using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)on pear fruits before and after larval feeding.Differential metabolites were screened and identified through multivariate statistical analyses.Furthermore,the effects of jasmonic acid(JA)and methyl jasmonate(MeJA)at different concentrations on larval feeding and female oviposition behaviors were examined.A total of 2 987 differential metabolites were identified,mainly including car-boxylic acids and their derivatives,organic oxygen compounds,and fatty acyls.Metabolic pathway enrichment analysis showed that the α-linolenic acid metabolic pathway exhibited the most significant changes,with 21 metabolites(α-linolenic acid,12-oxo phytodienoic acid,and 13-(S)-hydroperoxy-octadecatrienoic acid)being highly enriched,and the downstream signaling molecules JA and MeJA sig-nificantly upregulated.Feeding assays demonstrated that JA and MeJA significantly deterred larval feed-ing,particularly at 2 mmol/L,with combined JA+MeJA treatment showing even stronger inhibitory effects.Similarly,oviposition preference was significantly suppressed by JA or MeJA at 1 or 2 mmol/L,while a combination at 0.5 and 2 mmol/L yielded more pronounced effects.These results indicate that JA and MeJA,as key signaling molecules in the α-linolenic acid metabolic pathway,play vital roles in the induced defense response of pear fruit by effectively modulating larval feeding and adult oviposition behaviors of G.molesta.关键词
梨小食心虫/梨果/代谢组学/取食胁迫响应/次生代谢物/行为调控Key words
Grapholita molesta/pear fruit/metabolomics/herbivory stress response/secondary metabolite/behavioral regulation引用本文复制引用
刘昌念,陈航,申瑞威,赵育松,王怡,李捷,马瑞燕,孔维娜..梨小食心虫取食后梨果的代谢响应及茉莉酸类物质对其行为的调控作用[J].植物保护学报,2025,52(3):591-602,12.基金项目
国家现代农业(梨)产业技术体系(CARS-28),中央引导地方科技发展资金资助项目(YDZJSX2022A034),山西省重点研发计划(202302140601011),山西省科技创新人才团队专项(202304051001006),国家重点研发计划(2023YFD1401400) （梨）
