中国现代医生2025，Vol.63Issue(19)：12-15,41,5.DOI:10.3969/j.issn.1673-9701.2025.19.003
振荡电场刺激对大鼠脊髓损伤后内源性神经干细胞增殖及运动功能恢复的影响
Effects of oscillating field stimulation on the proliferation of endogenous neural stem cell and recovery of motor function in rats after spinal cord injury
摘要
Abstract
Objective To investigate the effec of oscillating field stimulation on the proliferation of endogenous neural stem cell(ENSC)and recovery of motor function after spinal cord injury(SCI)in rats.Methods The SCI model was established by using the improved Allen's strike method and was randomly divided into experimental group and control group,with 60 rats in each group.The experimental group received an oscillating field implant as the intervention,while the control group was equipped with the same device but without activation.After successful modeling,the spinal cord of rats was removed on the 3rd,7th and 14th day,respectively.The proliferation of ENSC was detected by neuroepithelial stem cell protein(Nestin)/5-bromodeoxyuridine(BrdU)immunofluorescence,and the expression of Wnt-3a protein in spinal cord was detected by immunohistochemistry.At the 4th,6th and 8th week,the spinal cord was stained with osmic acid to observe the formation of myelin sheath in rats,and Basso-Beattle-Bresnahan(BBB)functional score of each group of rats was performed before sampling.Results The immunofluorescence results showed that Nestin/BrdU double positive cells in the injured area could be seen in both groups on the 3rd day after SCI,reaching a peak on the 7th day,and gradually decreased on the 14th day,and still maintained at a high level.At the same time,number of Nestin/BrdU double positive cells in experimental group rats at each time point was more than that in control group.The immunohistochemical results showed that the expression level of Wnt-3a protein in experimental group rats at each time point was higher than that in control group(P＜0.05).At the 6th and 8th weeks,the number of myelin sheaths in experimental group rats was significantly higher than that in control group(P＜0.05).At the 4 th,6th and 8th weeks,the BBB scores of rats in experimental group were significantly higher than those in control group(P＜0.05).Conclusion The oscillating field stimulation can induce the proliferation of ENSC after SCI by activating Wnt/β-catenin signaling pathway,promote myelin regeneration,and improve motor function of rats.关键词
振荡电场刺激/脊髓损伤/内源性神经干细胞/Wnt-3a蛋白/髓鞘Key words
Oscillating field stimulation/Spinal cord injury/Endogenous neural stem cell/Wnt-3a protein/Myelin分类
医药卫生引用本文复制引用
张坤坤,邵晨,乔少林,仇大鹏,刘政劼..振荡电场刺激对大鼠脊髓损伤后内源性神经干细胞增殖及运动功能恢复的影响[J].中国现代医生,2025,63(19):12-15,41,5.基金项目
安徽省高等学校自然科学研究重点项目(2024AH051221) （2024AH051221）
蚌埠市科技创新类指导项目(2024ZD0038) （2024ZD0038）
